FIRST PUBLISH OCTOBER 2024
MCB 2610 Lab Practical 1 Exam Study
Guide Solutions
gram positive - ANSWER✔✔-purple cocci
gram negative - ANSWER✔✔-pink rods
endospore - ANSWER✔✔-responses to certain environmental conditions such as depletion of nutrients
stages of growth curve: - ANSWER✔✔-lag, exponential (log), stationary, death
lag phase - ANSWER✔✔-occurs when culture is transferred to fresh growth medium
exponential phase - ANSWER✔✔-growth is maximal and at a steady rate, culture is stable both
chemically and physically
stationary phase - ANSWER✔✔-cell division of new cell growth is matched by equal amount of cell death
death phase - ANSWER✔✔-caused by accumulation of toxic waste products, cell damage from exposure,
of chemicals in the environment
absorbance - ANSWER✔✔-change in optical density
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FIRST PUBLISH OCTOBER 2024
generation time - ANSWER✔✔-time required by populations to double in number
CHNOPS - ANSWER✔✔-carbon, hydrogen, nitrogen, oxygen, phosphorus, sulfur
liquid media - ANSWER✔✔-to grow large number or organisms (nutrient broth)
solid media - ANSWER✔✔-used to examine colony morphology of organisms and to help isolate pure
cultures
complex media - ANSWER✔✔-chemical composition is unknown
synthetic media - ANSWER✔✔-made of strict list of inorganic compounds and carbon sources
general purpose media - ANSWER✔✔-supports growth of wade range of organisms (nutrient broth,
nutrient agar, tryptic soy broth)
selective media - ANSWER✔✔-media that selects for a certain type of microbe by providing optimal
conditions for its growth
differential media - ANSWER✔✔-allow certain bacteria to be differentiated from others, typically by
change in color, production of clearing zones
exoenzymes - ANSWER✔✔-enzymes that are secreted by cells into their environment
3 common exoenzymes - ANSWER✔✔-amylase, caseinase, gelatinase
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