Medical Interventions EOC Test Questions And
Answers (A+)
Devise appropriate tests to determine the identity of the pathogen Answer BLAST
Be able to perform serial dilutions Answer yes
What are antigens Answer alert body of foreign object
What are antibodies? How are they made Answer fight against antigens; from plasma
cells
ELISA test steps Answer 1. Add sample, antigens bind to well
2. Wash
3. Add anti-bodies
4. Wash
5. Secondary antibody and enzyme bind
6. Wash
7. substrate added to produce color change w enzyme
when someone has been recently infected you want to test them for - Answer antigens
also know how to do concentration for ELISA - Answer again yeah
process of DNA sequencing: - Answer denature, add primers, DNA polymerase,
nucleotides, fluoresent nucleotides
, what is the purpose of PCR - Answer to make many many copies
what are primers and how are they used in PCR - Answer stop the sequence
when using PCR in DNA sequencing (cycle sequencing) how do dideoxynucleotides
differ from normal nucleotides added in the master mix - Answer fluorescent
what is happening when a dideoxynucleotide is added into a DNA strand during
replication? what does that mean in DNA sequencing - Answer stops sequence - helps
with gel electrophoresis
what is the purpose of gel electrophoresis in DNA sequencing - Answer to determine the
# of base pairs
capsule (bacterial structure) - Answer protects cell and acts as a barrier against WBC
cell wall (bacterial structure) - Answer semi-rigid structure
plasma membrane (bacterial structure) - Answer controls transport
flagella (bacterial structure) - Answer movement
ribosome (bacterial structure) - Answer makes proteins
storage granule (bacterial structure) - Answer store energy
nucleoid (bacterial structure) - Answer stores DNA
gram positive - Answer thick layer, dyed pink, more resistant to antibiotics
gram negative - Answer thin layer, purple, produce endotoxins
tetracyclines - Answer inhibit protein synthesis
sulfonamides - Answer inhibits folic acid synthesis
Answers (A+)
Devise appropriate tests to determine the identity of the pathogen Answer BLAST
Be able to perform serial dilutions Answer yes
What are antigens Answer alert body of foreign object
What are antibodies? How are they made Answer fight against antigens; from plasma
cells
ELISA test steps Answer 1. Add sample, antigens bind to well
2. Wash
3. Add anti-bodies
4. Wash
5. Secondary antibody and enzyme bind
6. Wash
7. substrate added to produce color change w enzyme
when someone has been recently infected you want to test them for - Answer antigens
also know how to do concentration for ELISA - Answer again yeah
process of DNA sequencing: - Answer denature, add primers, DNA polymerase,
nucleotides, fluoresent nucleotides
, what is the purpose of PCR - Answer to make many many copies
what are primers and how are they used in PCR - Answer stop the sequence
when using PCR in DNA sequencing (cycle sequencing) how do dideoxynucleotides
differ from normal nucleotides added in the master mix - Answer fluorescent
what is happening when a dideoxynucleotide is added into a DNA strand during
replication? what does that mean in DNA sequencing - Answer stops sequence - helps
with gel electrophoresis
what is the purpose of gel electrophoresis in DNA sequencing - Answer to determine the
# of base pairs
capsule (bacterial structure) - Answer protects cell and acts as a barrier against WBC
cell wall (bacterial structure) - Answer semi-rigid structure
plasma membrane (bacterial structure) - Answer controls transport
flagella (bacterial structure) - Answer movement
ribosome (bacterial structure) - Answer makes proteins
storage granule (bacterial structure) - Answer store energy
nucleoid (bacterial structure) - Answer stores DNA
gram positive - Answer thick layer, dyed pink, more resistant to antibiotics
gram negative - Answer thin layer, purple, produce endotoxins
tetracyclines - Answer inhibit protein synthesis
sulfonamides - Answer inhibits folic acid synthesis
