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Exam (elaborations)

MB (ASCP) () EXAM QUESTIONS WITH ANSWERS

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MB (ASCP) () EXAM QUESTIONS WITH ANSWERS

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MB (ASCP) (2024-2025) EXAM QUESTIONS WITH
ANSWERS

Which microorganism is detected by branched DNA
amplification? - <<<Answers>>>DNA or RNA


Which technique uses RNA, antibodies, and DNA? -
<<<Answers>>>Hybrid Capture Assay


Use of bisulfite treatment of DNA to determine its pattern of
methylation - <<<Answers>>>Bisulfite DNA
Multiplex PCR: short PCR products are weak -
<<<Answers>>>Decrease annealing time
Decrease annealing temperature
Decrease extension time
Decrease extension temperature
Increase KCl concentration
Decrease denaturing time


Multiplex PCR: longer pcr products are weak -
<<<Answers>>>Increase annealing time
Decrease annealing temperature
Increase extension time
Increase extension temperature
Decrease KCl concentration
Increase denaturing time

, MB (ASCP) (2024-2025) EXAM QUESTIONS WITH
ANSWERS

Metacentric - <<<Answers>>>


Submetacentric - <<<Answers>>>


Acrocentric - <<<Answers>>>


Telocentric - <<<Answers>>>


How many chromosomes during M Phase (end of mitosis)? -
<<<Answers>>>46 chromosomes


How many chromosomes during G1 Phase? -
<<<Answers>>>46 chromosomes


What happens during S Phase? - <<<Answers>>>DNA is
replicated


How many chromosomes during G2 Phase? -
<<<Answers>>>92 chromosomes


rRNA small subunit (prokaryotes) - <<<Answers>>>16s


rRNA large subunit (prokaryotes) - <<<Answers>>>23s & 5s

, MB (ASCP) (2024-2025) EXAM QUESTIONS WITH
ANSWERS

rRNA small subunit (eukaryotes) - <<<Answers>>>18s


rRNA large subunit (eukaryotes) - <<<Answers>>>5.8s &
28s


Long stretches of noncoding DNA in newly transcribed
mRNA - <<<Answers>>>Introns


Newly transcribed mRNA that contains introns which are
removed by splicing - <<<Answers>>>Heteronuclear RNA
(hnRNA)


Remaining sequences of mRNA that code for protein products
- <<<Answers>>>Exons


Phenotype will be present in individuals w/ one or both
chromosomes mutated - <<<Answers>>>Autosomal-
dominant


Likelihood of the disease phenotype in offspring is 25%
indicating the presence of the recessive mutation in at least
one chromosome in each of the parents -
<<<Answers>>>Autosomal-recessive
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