BOC Urinalysis / Body Fluids Questions
With Solutions
d (Measure the total volume of the sample before removing an aliquot. To calculate the total
protein, measure the protein of an aliquot to learn the mg/dL. Then multiply that answer by the
number of dL in the 24-hour collection.) 1) After receiving a 24 hr. urine for quantitative
total protein analysis the technician must first:
a. subculture the urine for bacteria
b. add the appropriate preservative
c. screen for albumin using a dipstick
d. measure the total volume
a (Urobilinogen is degraded by light.) 2) False results in the urobilinogen testing may
occur if the urine specimen is:
a. exposed to light
b. adjusted to a neutral pH
c. cooled to room temp
d. collected in a nonsterile container
b (It is common practice to share samples between the microbiology department and urinalysis.
Ideally, the culture is set up first to prevent contamination. If that is not feasible time wise, the
, BOC Urinalysis / Body Fluids Questions
With Solutions
sample should be aliquotted using aseptic technique, and refrigerated until it can be cultured.)
3) A clean catch urine is submitted to laboratory for routine urinalysis and culture. The
routine urinalysis is done first, and 3 hrs. later, the specimen is sent to the microbiology
department for culture. The specimen should:
a. be centrifuged, and the supernatant culture
b. be rejected due to the time delay
c. not be cultured if no bacteria are seen
d. be processed for culture only if the nitrate is positive
d (Bilirubin is degraded by light. The other analytes will not be affected.) 4) Which of the
following urine results is most apt to be changed by prolonged exposure to light?
a. pH
b. protein
c. ketones
d. bilirubin
c (Evaluate each statement. a is incorrect because these cells don't agglutinate. b is partially
correct, but urobilinogen decreases in light. c is true, bacterial overgrowth does lead to an
, BOC Urinalysis / Body Fluids Questions
With Solutions
alkaline urine. d is false, ketones are produced by fat metabolism in the patient.) 5) Urine
samples should be examined within 1 hr. of voiding because:
a. RBC's, leukocytes and casts agglutinate on standing for several hrs. at room temp
b. urobilinogen increases and bilirubin decreases after prolonged exposure to light
c. bacterial contamination will cause alkalinization of the urine
d. ketones will increase due to bacterial and cellular metabolism
Ketones 6) If a urine specimen was stored uncapped at 5 degrees C without preservation
and retested at 2pm which of the following test results would be changed due to these storage
conditions?
A. glucose
B. ketones
C. protein
D. nitrite
b (Storage must inhibit bacterial growth but not kill the bacteria. Freezing and additives are not
acceptable. The most commonly used method of preservation is refrigeration.) 7) A urine
specimen comes to the laboratory 7 hrs. after it is obtained. It is acceptable for culture only if the
specimen has been stored:
, BOC Urinalysis / Body Fluids Questions
With Solutions
a. at room temp
b. at 4 - 7 C
c. frozen
d. with a preservative additive
c (Consider whether a substance can increase or decrease outside the body. No more blood can
be produced. Although the RBC may rupture, they will still make a positive result on a
biochemical strip. The amount of solutes won't change, so specific gravity won't change. pH is
affected by metabolism of the urine components by bacteria, and room temperature is warm
enough for this to occur. Protein will not increase or decrease.) 8) Which of the following
would be affected by allowing a urine specimen to remain at room temp for 3 hrs. before
analysis:
a. occult blood
b. specific gravity
c. pH
d. protein
Daily void is approximately 1,500mL, but can range from 600-2,000mL
With Solutions
d (Measure the total volume of the sample before removing an aliquot. To calculate the total
protein, measure the protein of an aliquot to learn the mg/dL. Then multiply that answer by the
number of dL in the 24-hour collection.) 1) After receiving a 24 hr. urine for quantitative
total protein analysis the technician must first:
a. subculture the urine for bacteria
b. add the appropriate preservative
c. screen for albumin using a dipstick
d. measure the total volume
a (Urobilinogen is degraded by light.) 2) False results in the urobilinogen testing may
occur if the urine specimen is:
a. exposed to light
b. adjusted to a neutral pH
c. cooled to room temp
d. collected in a nonsterile container
b (It is common practice to share samples between the microbiology department and urinalysis.
Ideally, the culture is set up first to prevent contamination. If that is not feasible time wise, the
, BOC Urinalysis / Body Fluids Questions
With Solutions
sample should be aliquotted using aseptic technique, and refrigerated until it can be cultured.)
3) A clean catch urine is submitted to laboratory for routine urinalysis and culture. The
routine urinalysis is done first, and 3 hrs. later, the specimen is sent to the microbiology
department for culture. The specimen should:
a. be centrifuged, and the supernatant culture
b. be rejected due to the time delay
c. not be cultured if no bacteria are seen
d. be processed for culture only if the nitrate is positive
d (Bilirubin is degraded by light. The other analytes will not be affected.) 4) Which of the
following urine results is most apt to be changed by prolonged exposure to light?
a. pH
b. protein
c. ketones
d. bilirubin
c (Evaluate each statement. a is incorrect because these cells don't agglutinate. b is partially
correct, but urobilinogen decreases in light. c is true, bacterial overgrowth does lead to an
, BOC Urinalysis / Body Fluids Questions
With Solutions
alkaline urine. d is false, ketones are produced by fat metabolism in the patient.) 5) Urine
samples should be examined within 1 hr. of voiding because:
a. RBC's, leukocytes and casts agglutinate on standing for several hrs. at room temp
b. urobilinogen increases and bilirubin decreases after prolonged exposure to light
c. bacterial contamination will cause alkalinization of the urine
d. ketones will increase due to bacterial and cellular metabolism
Ketones 6) If a urine specimen was stored uncapped at 5 degrees C without preservation
and retested at 2pm which of the following test results would be changed due to these storage
conditions?
A. glucose
B. ketones
C. protein
D. nitrite
b (Storage must inhibit bacterial growth but not kill the bacteria. Freezing and additives are not
acceptable. The most commonly used method of preservation is refrigeration.) 7) A urine
specimen comes to the laboratory 7 hrs. after it is obtained. It is acceptable for culture only if the
specimen has been stored:
, BOC Urinalysis / Body Fluids Questions
With Solutions
a. at room temp
b. at 4 - 7 C
c. frozen
d. with a preservative additive
c (Consider whether a substance can increase or decrease outside the body. No more blood can
be produced. Although the RBC may rupture, they will still make a positive result on a
biochemical strip. The amount of solutes won't change, so specific gravity won't change. pH is
affected by metabolism of the urine components by bacteria, and room temperature is warm
enough for this to occur. Protein will not increase or decrease.) 8) Which of the following
would be affected by allowing a urine specimen to remain at room temp for 3 hrs. before
analysis:
a. occult blood
b. specific gravity
c. pH
d. protein
Daily void is approximately 1,500mL, but can range from 600-2,000mL
