©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
ASCP CG Certification Practice Exam
Questions and Answers 100% Pass
What is the best agent to use against mycoplasma? - ✔✔BM cyclin - a
combination of two antibiotics (tiamulin and minocycline) that effecticely
targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be
evaluated by: - ✔✔comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - ✔✔Proteolytic enzymes - break
down tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with: - ✔✔Colcemid - mitotic spindle poison
that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - ✔✔Logged in
to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
✔✔T-cell lymphocytes
,©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document? - ✔✔Expiration
dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus)
sample? - ✔✔Dissection
Which cell type is most problematic if found in a CVS (chorionic villus)
sample? - ✔✔Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of
cytoplasm is still seen around the mets, what is the most likely cause? -
✔✔innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? - ✔✔Asceptic technique
When there are not enough mets to complete the study of a patient/case,
what needs to be done next? - ✔✔prepare additional slides from any
remaining sample
When looking at a dropped slide under a phase scope and mets are very
spread out and sparse, what could help fix the issue? - ✔✔Increading the
drying time by using warm slides
, ©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
What is true of an inverted microscope? - ✔✔the objectives are beneath
the stage
High-resolution banding is indicated for a possible diagnosis of: -
✔✔DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent),
tubular nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what
is the MOST important data to accompany the specimen? - ✔✔Reason for
referral
Which type of specimen should be requested for a cytogenetic analysis for
turner syndrome? - ✔✔Heparinized peripheral blood stored at room
temperature
(NOT FROZEN)
ASCP CG Certification Practice Exam
Questions and Answers 100% Pass
What is the best agent to use against mycoplasma? - ✔✔BM cyclin - a
combination of two antibiotics (tiamulin and minocycline) that effecticely
targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be
evaluated by: - ✔✔comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - ✔✔Proteolytic enzymes - break
down tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with: - ✔✔Colcemid - mitotic spindle poison
that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - ✔✔Logged in
to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
✔✔T-cell lymphocytes
,©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document? - ✔✔Expiration
dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus)
sample? - ✔✔Dissection
Which cell type is most problematic if found in a CVS (chorionic villus)
sample? - ✔✔Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of
cytoplasm is still seen around the mets, what is the most likely cause? -
✔✔innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? - ✔✔Asceptic technique
When there are not enough mets to complete the study of a patient/case,
what needs to be done next? - ✔✔prepare additional slides from any
remaining sample
When looking at a dropped slide under a phase scope and mets are very
spread out and sparse, what could help fix the issue? - ✔✔Increading the
drying time by using warm slides
, ©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
What is true of an inverted microscope? - ✔✔the objectives are beneath
the stage
High-resolution banding is indicated for a possible diagnosis of: -
✔✔DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent),
tubular nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what
is the MOST important data to accompany the specimen? - ✔✔Reason for
referral
Which type of specimen should be requested for a cytogenetic analysis for
turner syndrome? - ✔✔Heparinized peripheral blood stored at room
temperature
(NOT FROZEN)
