PCB 3233: Immunology; Exam 2-
Chapters 3 & 4
When a mannose receptor is engaged what mechanism is initiated in a phagocyte? - correct answer
✔✔lectin complement
When CR3 & CR4 receptors are engaged what mechanism is initiated in a phagocyte? - correct answer
✔✔iC3b; LPS
When scavenger receptors are engaged what mechanism is initiated in a phagocyte? - correct answer
✔✔negatively charged molecules: nucleic acids, LPS, lipoteichoic acids
When the following TLRs are engaged what mechanism is initiated in a macrophage? - correct answer
✔✔cytokine creation and secretion
What does TLR-3 bind to? - correct answer ✔✔double-stranded viral RNA
What does TLR-4 bind to? - correct answer ✔✔gram-negative bacteria: LPS
What does TLR-5 bind to? - correct answer ✔✔bacteria falgellin
What does TLR-7 bind to? - correct answer ✔✔single-stranded viral RNA
TLR Signaling Mechanism - correct answer ✔✔1. a complex of TLR-4, MD2, CD14, and LPS assembles on
a macrophage surface
2. MyD88 binds to TLR4; IRAK4 binds to TRAF6, phosphorylates IKK
3. IKK phosphorylates IkB, releases NFkB
4. NFkB activates inflammatory cytokines
, What 5 genes are transcribed because of TLR-4 recognition? - correct answer ✔✔IL-1β, TNF-α, IL-6,
CXCL-8 (IL-8), IL-12
What is the general term given to the 5 gene products that are transcribed because of TLR-4
recognition? - correct answer ✔✔inflammatory cytokines
What do neutrophils do? - correct answer ✔✔phagocytosis
What is the lineage of neutrophils? - correct answer ✔✔hematopoietic stem cell-common myeloid
precursor-granulocyte macrophage progenitor-neutrophil
What is another name for neutrophils? - correct answer ✔✔polymorphonuclear leukocyte
What are the 4 classes of adhesion molecules? - correct answer ✔✔selectins, vascular addressins,
intergrins, immunoglobulin superfamily
What do selectins bind to? - correct answer ✔✔carbohydrates
What do vascular addressins bind to? - correct answer ✔✔L-selectins
What do integrins bind to? - correct answer ✔✔ICAM-1 (ig superfamily)
What does immunoglobulin superfamily bind to? - correct answer ✔✔LAF-1 (integrin)
What are the 4 steps of extravasation? - correct answer ✔✔1. rolling adhesion
2. tight binding
3. diapedesis
4. migration
Chapters 3 & 4
When a mannose receptor is engaged what mechanism is initiated in a phagocyte? - correct answer
✔✔lectin complement
When CR3 & CR4 receptors are engaged what mechanism is initiated in a phagocyte? - correct answer
✔✔iC3b; LPS
When scavenger receptors are engaged what mechanism is initiated in a phagocyte? - correct answer
✔✔negatively charged molecules: nucleic acids, LPS, lipoteichoic acids
When the following TLRs are engaged what mechanism is initiated in a macrophage? - correct answer
✔✔cytokine creation and secretion
What does TLR-3 bind to? - correct answer ✔✔double-stranded viral RNA
What does TLR-4 bind to? - correct answer ✔✔gram-negative bacteria: LPS
What does TLR-5 bind to? - correct answer ✔✔bacteria falgellin
What does TLR-7 bind to? - correct answer ✔✔single-stranded viral RNA
TLR Signaling Mechanism - correct answer ✔✔1. a complex of TLR-4, MD2, CD14, and LPS assembles on
a macrophage surface
2. MyD88 binds to TLR4; IRAK4 binds to TRAF6, phosphorylates IKK
3. IKK phosphorylates IkB, releases NFkB
4. NFkB activates inflammatory cytokines
, What 5 genes are transcribed because of TLR-4 recognition? - correct answer ✔✔IL-1β, TNF-α, IL-6,
CXCL-8 (IL-8), IL-12
What is the general term given to the 5 gene products that are transcribed because of TLR-4
recognition? - correct answer ✔✔inflammatory cytokines
What do neutrophils do? - correct answer ✔✔phagocytosis
What is the lineage of neutrophils? - correct answer ✔✔hematopoietic stem cell-common myeloid
precursor-granulocyte macrophage progenitor-neutrophil
What is another name for neutrophils? - correct answer ✔✔polymorphonuclear leukocyte
What are the 4 classes of adhesion molecules? - correct answer ✔✔selectins, vascular addressins,
intergrins, immunoglobulin superfamily
What do selectins bind to? - correct answer ✔✔carbohydrates
What do vascular addressins bind to? - correct answer ✔✔L-selectins
What do integrins bind to? - correct answer ✔✔ICAM-1 (ig superfamily)
What does immunoglobulin superfamily bind to? - correct answer ✔✔LAF-1 (integrin)
What are the 4 steps of extravasation? - correct answer ✔✔1. rolling adhesion
2. tight binding
3. diapedesis
4. migration
