CH 11/13/14/15 PCB 3233 Final
If you got multiple immunizations with the same antigen, what would you find with regard to Ab levels
(IgG) and antibody affinity? - correct answer ✔✔IgG concentration would increase after each
immunization
Affinity would increase
What is the difference between protective immunity and a secondary immune response? What
cells/molecules are used in each? - correct answer ✔✔Protective immunity occurs immediately after a
primary immune response (IR) whereas a secondary immune response occurs following a second
exposure to the same antigen
Protective immunity uses the residual antibodies from the primary IR
Secondary immune response uses memory cells
Do naïve B-cells help in a secondary immune response? What about a primary immune response? -
correct answer ✔✔No, inhibitory Fc-gamma-RIIB1 receptor
Yes
Do B-cells go through the same types of processes during a secondary immune response as they do
during a primary immune response? - correct answer ✔✔Yes → somatic hypermutation and affinity
maturation
Do we typically have more memory B-cells specific for antigen in a secondary IR than we had naïve B-cell
(antigen specific) in the primary response? - correct answer ✔✔yes
Know what original antigenic sin is and how to use the concept if you are asked a clinical type of
question. Can a naïve B-cell be activated in a secondary immune response? Can we use this knowledge
to help us in things like hemolytic disease of the newborn (how does RhoGAM work)? - correct answer
✔✔A naive B cell can NOT be activated in a secondary IR because of the Fc-gamma-RIIB1 receptor
1st pregnancy with Rh- mother and Rh+ baby: the fetal RBC binds to and activates the maternal B cell to
secrete igM plus low amounts of low-affinity IgG → some fetal RBC destruction → health baby
, 2nd pregnancy with Rh- mother and Rh+ baby: fetal RBC binds to and activates the maternal B cell to
secrete high amounts of high-affinity IgG → massive fetal RBC destruction → anemic baby
Pregnancies with Rh- mother (with RhoGAM) and Rh+ baby: RhoGAM binds to the fetal RBC and then
the FC-gamma-RIIB1 receptor to inhibit primary IR → no fetal RBC destruction → healthy baby
How long does it take for a primary IR vs. a secondary IR? - correct answer ✔✔Primary IR: 8 days
Secondary IR: 4 days
Which T-cells decline in an HIV patient? What is the significant # of those cells for a transition to AIDs? -
correct answer ✔✔CD4 T cells
<200 CD4 T cells per microliter of blood, then HIV will progress to AIDS
Know gp120 and gp41 and what they do? What is the precursor polypeptide these are created from? -
correct answer ✔✔Used by HIV virus Precursor → gp160
gp120 → binding
gp41 → fusion
What are tat and what is the function of each? Are all of these brought (premade) by the virus when it
infects the cell or are some made after the cell is infected? - correct answer ✔✔Tat → proteins bind to
the transcription activation region of the viral mRNA and prevents transcription from shutting off and
therefore increasing transcription of viral RNA
made after infection
What are rev and what is the function? Are all of these brought (premade) by the virus when it infects
the cell or are some made after the cell is infected? - correct answer ✔✔Rev → proteins control the
supply of viral RNA to the cytoplasm that are necessary for encoding proteins needed to make virions
made after infection
What are integrase and what is the function? Are all of these brought (premade) by the virus when it
infects the cell or are some made after the cell is infected? - correct answer ✔✔Integrase → integrates
the viral ds-cDNA into the host DNA
brought with the virus
If you got multiple immunizations with the same antigen, what would you find with regard to Ab levels
(IgG) and antibody affinity? - correct answer ✔✔IgG concentration would increase after each
immunization
Affinity would increase
What is the difference between protective immunity and a secondary immune response? What
cells/molecules are used in each? - correct answer ✔✔Protective immunity occurs immediately after a
primary immune response (IR) whereas a secondary immune response occurs following a second
exposure to the same antigen
Protective immunity uses the residual antibodies from the primary IR
Secondary immune response uses memory cells
Do naïve B-cells help in a secondary immune response? What about a primary immune response? -
correct answer ✔✔No, inhibitory Fc-gamma-RIIB1 receptor
Yes
Do B-cells go through the same types of processes during a secondary immune response as they do
during a primary immune response? - correct answer ✔✔Yes → somatic hypermutation and affinity
maturation
Do we typically have more memory B-cells specific for antigen in a secondary IR than we had naïve B-cell
(antigen specific) in the primary response? - correct answer ✔✔yes
Know what original antigenic sin is and how to use the concept if you are asked a clinical type of
question. Can a naïve B-cell be activated in a secondary immune response? Can we use this knowledge
to help us in things like hemolytic disease of the newborn (how does RhoGAM work)? - correct answer
✔✔A naive B cell can NOT be activated in a secondary IR because of the Fc-gamma-RIIB1 receptor
1st pregnancy with Rh- mother and Rh+ baby: the fetal RBC binds to and activates the maternal B cell to
secrete igM plus low amounts of low-affinity IgG → some fetal RBC destruction → health baby
, 2nd pregnancy with Rh- mother and Rh+ baby: fetal RBC binds to and activates the maternal B cell to
secrete high amounts of high-affinity IgG → massive fetal RBC destruction → anemic baby
Pregnancies with Rh- mother (with RhoGAM) and Rh+ baby: RhoGAM binds to the fetal RBC and then
the FC-gamma-RIIB1 receptor to inhibit primary IR → no fetal RBC destruction → healthy baby
How long does it take for a primary IR vs. a secondary IR? - correct answer ✔✔Primary IR: 8 days
Secondary IR: 4 days
Which T-cells decline in an HIV patient? What is the significant # of those cells for a transition to AIDs? -
correct answer ✔✔CD4 T cells
<200 CD4 T cells per microliter of blood, then HIV will progress to AIDS
Know gp120 and gp41 and what they do? What is the precursor polypeptide these are created from? -
correct answer ✔✔Used by HIV virus Precursor → gp160
gp120 → binding
gp41 → fusion
What are tat and what is the function of each? Are all of these brought (premade) by the virus when it
infects the cell or are some made after the cell is infected? - correct answer ✔✔Tat → proteins bind to
the transcription activation region of the viral mRNA and prevents transcription from shutting off and
therefore increasing transcription of viral RNA
made after infection
What are rev and what is the function? Are all of these brought (premade) by the virus when it infects
the cell or are some made after the cell is infected? - correct answer ✔✔Rev → proteins control the
supply of viral RNA to the cytoplasm that are necessary for encoding proteins needed to make virions
made after infection
What are integrase and what is the function? Are all of these brought (premade) by the virus when it
infects the cell or are some made after the cell is infected? - correct answer ✔✔Integrase → integrates
the viral ds-cDNA into the host DNA
brought with the virus
