Blood bank immunohematology Review Questions with complete Solutions Graded A+
The loss of ATP leads to - Answers A decrease in phosphorylation of spectrin and loss of membrane
deformability
The majority of normal adult hemoglobin consists of - Answers 2 alpha chains and 2 beta chains
When RBC's are stored, there is a "shift to the left" which means - Answers Hemoglobin oxygen affinity
increases, owing to a decrease in 2,3-DPG.
What is the role of platelets (3 things) - Answers Maintain vascular integrity
initial arrest of bleeding
stabilizing of hemostatic plug
What anticoagulant-preservatives provide a storage time of 21 days at 1-6degreesC for whole blood and
prepared RBC's if an additive solution is not added? - Answers ACD
CP2D
CPD
What is current storage time and temp for platelet concentrates and apheresis platelet components? -
Answers 5 days at 20-24 degrees C
What is the minimum number of platelets required in a platelet concentrate prepared from whole blood
by centrifugation (90% of sampled units)? - Answers 5.5 x 10^11
What factors will influence platelet metabolism and viability in a closed container system? - Answers
Total platelet count,
duration of storage,
temperature of storage.
NOT fibrinogen concentration
What is the minimum number of platelets required in an apheresis component (90% of sampled units)? -
Answers 3 x 10^11
Whole blood and RBC units are stored at what temperature? - Answers 1-6degrees C
Additive solutions are approved for blood storage for how many days? - Answers 42
Whole blood/RBC's can be stored in CPDA-1 at 1-6degrees for how many days? - Answers 35
What is the lowest allowable pH for a platelet component at outdate? - Answers 6.2
,Frozen/thawed RBC's processed in an open system can be stored for how long? - Answers 24 hours
What is the hemoglobin source for hemoglobin-based RBC substitutes in advanced clinical testing. -
Answers Both bovine and human hemoglobins.
Meiosis - Answers Genetic material is duplicated, equally divided between 2 daughter cells.
When a recessive trait is expressed, it means that - Answers Two genes carrying the trait were present.
In a pedigree, the "index case" is another name for - Answers Propositus
What 4 nitrogenous bases make up DNA? - Answers Adenine, cytosine, guanine, thymine.
Proteins and peptides are composed of - Answers linear arrangements of amino acids.
Which phenotype could not result from the mating of a
Jk(a+b+) female and a Jk(a+b+) male - Answers Jk(a-b-)
Exon - Answers The coding region of a gene.
PCR technology can be used to - Answers amplify small amounts of DNA.
Transcription - Answers Synthesis of RNA using DNA as a template.
When a man possesses a phenotypic trait he passes it to all his daughters and none of his sons, the trait
is: - Answers X-linked dominant
When a woman possesses a phenotypic trait that she passes to all of her sons and none of her
daughters, it is - Answers X-linked recessive
DNA is replicated - Answers Semiconservatively from DNA
RNA is processed - Answers After RNA is copied from DNA
Translation of proteins from RNA takes place - Answers Inside the nucleolus of the cell
Meiosis is necessary to - Answers generate new DNA sequences in daughter cells.
Characteristic of the acquired IS include - Answers production of antibody and lymphokines
Substances that participate in the regulation of the immune response. - Answers Cytokines
What cell is identified primarily by the presence of immunoglobulin on its surface? - Answers B cell
Which cell functions primarily in antigen processing and presentation? - Answers Macrophage
Which is the signal for the expression of IL-2 receptors on a TH cell? - Answers Presentation of antigen
by a monocyte to a TH cell.
, Which class of HLA markers are important in immune recognition of antigen in cell-to-cell interactions? -
Answers Class II
Which immunoglobulin class would be most important in the study of immune-mediated transfusion
reactions due to the presence of alloantibodies? - Answers IgG
Which immunoglobulins can activate the classic pathway of the complement system? - Answers IgG1,
IgG3, IgM
Which complement factor is common to both classic and alternative pathways and will result in the
formation of MAC? - Answers Factor C3
Which of the following patient samples for blood bank testing is most likely to contain active
complement?
*EDTA sample
*Heparin sample
*Serum sample drawn 2 days ago
*Serum sample drawn 2 hours ago - Answers Serum sample drawn 2 hours ago
Which one of the following statements is true concerning the formation of antibodies to blood group
antigens?
*All blood group antigens are immunogenic
*Only ABO and Rh group antigens are immunogenic
*Antibodies may form against any foreign blood group antigen
*Antibodies formed against Kidd antigens are always IgM - Answers Antibodies may form against any
foreign blood group antigen
Antibody idiotype is determined by the - Answers Variable regions of heavy and light chains.
What antibody is produced rapidly and in the highest amounts during secondary response? - Answers
IgG
Which of the following is mostly likely to account for the Failure of a visible RBC antigen-antibody
reaction?
*Homozygous expression of an antigen on an RBC
*pH of 7.2
*Incubation at 37degrees for suspended IgG antibody
The loss of ATP leads to - Answers A decrease in phosphorylation of spectrin and loss of membrane
deformability
The majority of normal adult hemoglobin consists of - Answers 2 alpha chains and 2 beta chains
When RBC's are stored, there is a "shift to the left" which means - Answers Hemoglobin oxygen affinity
increases, owing to a decrease in 2,3-DPG.
What is the role of platelets (3 things) - Answers Maintain vascular integrity
initial arrest of bleeding
stabilizing of hemostatic plug
What anticoagulant-preservatives provide a storage time of 21 days at 1-6degreesC for whole blood and
prepared RBC's if an additive solution is not added? - Answers ACD
CP2D
CPD
What is current storage time and temp for platelet concentrates and apheresis platelet components? -
Answers 5 days at 20-24 degrees C
What is the minimum number of platelets required in a platelet concentrate prepared from whole blood
by centrifugation (90% of sampled units)? - Answers 5.5 x 10^11
What factors will influence platelet metabolism and viability in a closed container system? - Answers
Total platelet count,
duration of storage,
temperature of storage.
NOT fibrinogen concentration
What is the minimum number of platelets required in an apheresis component (90% of sampled units)? -
Answers 3 x 10^11
Whole blood and RBC units are stored at what temperature? - Answers 1-6degrees C
Additive solutions are approved for blood storage for how many days? - Answers 42
Whole blood/RBC's can be stored in CPDA-1 at 1-6degrees for how many days? - Answers 35
What is the lowest allowable pH for a platelet component at outdate? - Answers 6.2
,Frozen/thawed RBC's processed in an open system can be stored for how long? - Answers 24 hours
What is the hemoglobin source for hemoglobin-based RBC substitutes in advanced clinical testing. -
Answers Both bovine and human hemoglobins.
Meiosis - Answers Genetic material is duplicated, equally divided between 2 daughter cells.
When a recessive trait is expressed, it means that - Answers Two genes carrying the trait were present.
In a pedigree, the "index case" is another name for - Answers Propositus
What 4 nitrogenous bases make up DNA? - Answers Adenine, cytosine, guanine, thymine.
Proteins and peptides are composed of - Answers linear arrangements of amino acids.
Which phenotype could not result from the mating of a
Jk(a+b+) female and a Jk(a+b+) male - Answers Jk(a-b-)
Exon - Answers The coding region of a gene.
PCR technology can be used to - Answers amplify small amounts of DNA.
Transcription - Answers Synthesis of RNA using DNA as a template.
When a man possesses a phenotypic trait he passes it to all his daughters and none of his sons, the trait
is: - Answers X-linked dominant
When a woman possesses a phenotypic trait that she passes to all of her sons and none of her
daughters, it is - Answers X-linked recessive
DNA is replicated - Answers Semiconservatively from DNA
RNA is processed - Answers After RNA is copied from DNA
Translation of proteins from RNA takes place - Answers Inside the nucleolus of the cell
Meiosis is necessary to - Answers generate new DNA sequences in daughter cells.
Characteristic of the acquired IS include - Answers production of antibody and lymphokines
Substances that participate in the regulation of the immune response. - Answers Cytokines
What cell is identified primarily by the presence of immunoglobulin on its surface? - Answers B cell
Which cell functions primarily in antigen processing and presentation? - Answers Macrophage
Which is the signal for the expression of IL-2 receptors on a TH cell? - Answers Presentation of antigen
by a monocyte to a TH cell.
, Which class of HLA markers are important in immune recognition of antigen in cell-to-cell interactions? -
Answers Class II
Which immunoglobulin class would be most important in the study of immune-mediated transfusion
reactions due to the presence of alloantibodies? - Answers IgG
Which immunoglobulins can activate the classic pathway of the complement system? - Answers IgG1,
IgG3, IgM
Which complement factor is common to both classic and alternative pathways and will result in the
formation of MAC? - Answers Factor C3
Which of the following patient samples for blood bank testing is most likely to contain active
complement?
*EDTA sample
*Heparin sample
*Serum sample drawn 2 days ago
*Serum sample drawn 2 hours ago - Answers Serum sample drawn 2 hours ago
Which one of the following statements is true concerning the formation of antibodies to blood group
antigens?
*All blood group antigens are immunogenic
*Only ABO and Rh group antigens are immunogenic
*Antibodies may form against any foreign blood group antigen
*Antibodies formed against Kidd antigens are always IgM - Answers Antibodies may form against any
foreign blood group antigen
Antibody idiotype is determined by the - Answers Variable regions of heavy and light chains.
What antibody is produced rapidly and in the highest amounts during secondary response? - Answers
IgG
Which of the following is mostly likely to account for the Failure of a visible RBC antigen-antibody
reaction?
*Homozygous expression of an antigen on an RBC
*pH of 7.2
*Incubation at 37degrees for suspended IgG antibody
