PCOL 440 Exam 2 Questions And 100% Correct Answers
Polyclonal antibodies are derived from a single clone of antibody-producing immune
cells. - ANSWER False
The active agents in snake bite antivenoms are antibodies. - ANSWER True
Monoclonal antibodies recognize multiple epitopes of a common antigen. - ANSWER
False
What is FALSE regarding Hybridoma technology?
- Allow the investigation of the detailed distribution of an antigen
- Allow diagnostic typing of diseases
- Allow therapy of human disease
can produce gene therapy vectors
can purify a substance to high degree - ANSWER can produce gene therapy vectors
Hybridoma technology developed for producing polyclonal antibodies. - ANSWER False
Concerning early pregnancy tests (EPT) select the FALSE statement.
antibodies are used to detect Human Chorionic Gonadotropin (hCG)
DNA probes are used to detect Human Chorionic Gonadotropin (hCG)
ELISA detects Human Chorionic Gonadotropin (hCG) It is crucial in pregnancy
Production of Human Chorionic Gonadotropin (hCG) is one of the early pregnancy
events DNA probes detect the presence of Human Chorionic Gonadotropin (hCG). Name
the following laboratory technique that does not make use of monoclonal antibodies
Southern blot
,Polyclonal antibodies are mixtures of antibodies that recognize distinct epitopes of the
antigen in question. - ANSWER True
NIH has requirements for scientific rigor and reproducibility that grantees are expected
to adhere to - ANSWER True
Monoclonal antibodies are derived from a single clone of antibody producing immune
cells - ANSWER True
Which of the following is FALSE
ELISA stands for enzyme linked immunosorbent assay
ELISAs are utilized to detect antibody-antigen interactions
- During an ELISA, experimental signal is detected through substrate catalysis to a
colored product by an enzyme linked to the antibody
- ELISA assays have no clinical utility whatsoever and are only being applied in
fundamental research laboratories
- Any molecule or molecular structure that stimulates the immune response is known as
antigens - CORRECT ANSWER ELISA assays have no clinical utility whatsoever and are
applied only in fundamental research laboratories
Using the allelic frequencies of the various STR loci in the associated table, calculate
the likelihood of an individual within the population having the following genotype.
Calculations performed using 2 significant figures:
D7S820: 11,11
F13A01: 6,7
LPL: 10,11 - ANSWER ~ 1 in 1,000
,Using the allelic frequencies of the various STR loci in the associated table, calculate
the likelihood of an individual within the population having the following genotype. (2
pts)
D7S820: 7, 10
THO1: 7, 9.3
TPOX: 8, 8
Calculations made using 2 significant figures: - ANSWER ~1 in 3,000
Antibodies can be used therapeutically to provide passive immunity to
- Alzheimer's Disease
- Snake bites
- Infectious diseases
- Diabetes
- Cancer - ANSWER Snake bites
Infectious diseases
The estimated cost of misidentified cell lines in biomedical research is approximately: -
ANSWER $1 billion
Cell strains are Immortal - ANSWER False
Cell Lines are Immortal - ANSWER True
Which of the following statements is NOT correct? ANSWER STR analysis can yield
absolute proof in civil and criminal cases
Any molecule or molecular structure that can elicit the immune response is called an
, antigen. ANSWER True
Which of the following statement is/are NOT true?
Antibodies are another name for immunoglobulin
There are five antibody classes
Antibodies exhibit extreme diversity
Antibodies are produced by T-cells
Antibodies have a therapeutic application ANSWER Antibodies are produced by T-cells
Antibodies can be used to provide passive immunity to infectious disease. - ANSWER
True
A karyotype is - ANSWER the characterization of the chromosomal complement of an
individual
Which technology did Dr. Stanley Gartler use to first note and publish about
misidentified cell lines in biomedical research - ANSWER Isoenzyme analysis
Established cell lines may undergo genetic evolution while grown in cell culture -
ANSWER True
Cell line authentication may be required for scientific manuscript and grant submissions
- ANSWER True
Which DNA marker is used to determine the sex of an individual from which a cell line is
derived? - ANSWER AMEL
STR analysis quantifies variation in the - ANSWER DNA size
Polyclonal antibodies are derived from a single clone of antibody-producing immune
cells. - ANSWER False
The active agents in snake bite antivenoms are antibodies. - ANSWER True
Monoclonal antibodies recognize multiple epitopes of a common antigen. - ANSWER
False
What is FALSE regarding Hybridoma technology?
- Allow the investigation of the detailed distribution of an antigen
- Allow diagnostic typing of diseases
- Allow therapy of human disease
can produce gene therapy vectors
can purify a substance to high degree - ANSWER can produce gene therapy vectors
Hybridoma technology developed for producing polyclonal antibodies. - ANSWER False
Concerning early pregnancy tests (EPT) select the FALSE statement.
antibodies are used to detect Human Chorionic Gonadotropin (hCG)
DNA probes are used to detect Human Chorionic Gonadotropin (hCG)
ELISA detects Human Chorionic Gonadotropin (hCG) It is crucial in pregnancy
Production of Human Chorionic Gonadotropin (hCG) is one of the early pregnancy
events DNA probes detect the presence of Human Chorionic Gonadotropin (hCG). Name
the following laboratory technique that does not make use of monoclonal antibodies
Southern blot
,Polyclonal antibodies are mixtures of antibodies that recognize distinct epitopes of the
antigen in question. - ANSWER True
NIH has requirements for scientific rigor and reproducibility that grantees are expected
to adhere to - ANSWER True
Monoclonal antibodies are derived from a single clone of antibody producing immune
cells - ANSWER True
Which of the following is FALSE
ELISA stands for enzyme linked immunosorbent assay
ELISAs are utilized to detect antibody-antigen interactions
- During an ELISA, experimental signal is detected through substrate catalysis to a
colored product by an enzyme linked to the antibody
- ELISA assays have no clinical utility whatsoever and are only being applied in
fundamental research laboratories
- Any molecule or molecular structure that stimulates the immune response is known as
antigens - CORRECT ANSWER ELISA assays have no clinical utility whatsoever and are
applied only in fundamental research laboratories
Using the allelic frequencies of the various STR loci in the associated table, calculate
the likelihood of an individual within the population having the following genotype.
Calculations performed using 2 significant figures:
D7S820: 11,11
F13A01: 6,7
LPL: 10,11 - ANSWER ~ 1 in 1,000
,Using the allelic frequencies of the various STR loci in the associated table, calculate
the likelihood of an individual within the population having the following genotype. (2
pts)
D7S820: 7, 10
THO1: 7, 9.3
TPOX: 8, 8
Calculations made using 2 significant figures: - ANSWER ~1 in 3,000
Antibodies can be used therapeutically to provide passive immunity to
- Alzheimer's Disease
- Snake bites
- Infectious diseases
- Diabetes
- Cancer - ANSWER Snake bites
Infectious diseases
The estimated cost of misidentified cell lines in biomedical research is approximately: -
ANSWER $1 billion
Cell strains are Immortal - ANSWER False
Cell Lines are Immortal - ANSWER True
Which of the following statements is NOT correct? ANSWER STR analysis can yield
absolute proof in civil and criminal cases
Any molecule or molecular structure that can elicit the immune response is called an
, antigen. ANSWER True
Which of the following statement is/are NOT true?
Antibodies are another name for immunoglobulin
There are five antibody classes
Antibodies exhibit extreme diversity
Antibodies are produced by T-cells
Antibodies have a therapeutic application ANSWER Antibodies are produced by T-cells
Antibodies can be used to provide passive immunity to infectious disease. - ANSWER
True
A karyotype is - ANSWER the characterization of the chromosomal complement of an
individual
Which technology did Dr. Stanley Gartler use to first note and publish about
misidentified cell lines in biomedical research - ANSWER Isoenzyme analysis
Established cell lines may undergo genetic evolution while grown in cell culture -
ANSWER True
Cell line authentication may be required for scientific manuscript and grant submissions
- ANSWER True
Which DNA marker is used to determine the sex of an individual from which a cell line is
derived? - ANSWER AMEL
STR analysis quantifies variation in the - ANSWER DNA size
