med lab midterm 1 review exam //All Chapters
With 100% Correct Answers / A + Grade.
Specifications (printed on the stem of the pipette): - CORRECT ANSWERS-Maximum
volume that can be transferred
Size of divisions on the pipette
What is the smallest volume that can be delivered accurately in this pipette? -
CORRECT ANSWERS-0.1 mL
Glass Pipettes - CORRECT ANSWERS-Volumetric (transfer) vs. Graduated
(measuring)
volumetric pipet - CORRECT ANSWERS-Calibrated to deliver a fixed volume of liquid
by drainage
Calibration Mark etched on stem, "bulb" in middle, gradual taper at tip
To be used when accuracy is required to deliver an exact amount
For ex: a 5 mL Volumetric pipette has a tolerance of 0.01 mL
Ostwald Pipette - CORRECT ANSWERS-similar to volumetric but with bulb closer to the
tip. Used for viscous fluids (ex. serum)
Graduated - CORRECT ANSWERS-Calibrated to deliver a volume of liquid between 2
calibration marks
an use to transfer various volumes
To be used when speed is required
Less accurate than volumetric pipettes
Ex: a 5 mL Graduated pipette has a tolerance of 0.02 mL
Two types of graduated pipettes - CORRECT ANSWERS-Mohr pipet - CORRECT
ANSWERS-Precipitate (pellet): the packed particles (solid) at the bottom of a
centrifuged tube
Supernatant: the liquid portion at the top of a centrifuged tube
,Decantation: separation of the supernatant from the pellet
- CORRECT ANSWERS-Rotor and carriers attached to the vertical shaft of a motor
and enclosed in a metal covering
Centrifuge: - CORRECT ANSWERS-apparatus used to separate substances of different
density or particle size, when suspended in a fluid, by spinning them about an axis in a
suitable container
Rotor and carriers attached to the vertical shaft of a motor and enclosed in a metal
covering
Rotor (centrifuge head): - CORRECT ANSWERS-Primary component of a centrifuge
which holds the material to be centrifuged and which is rotated by the drive system.
Centrifuges classified according to the following: - CORRECT ANSWERS-Benchtop vs.
floor
Refrigerated or not refrigerated
Rotor head type (fixed, hematocrit, swinging-bucket, angled)
Maximum speed attainable
Separating serum or plasma from blood cells
Normal separation of serum from clot: - CORRECT ANSWERS-Min of 1000 g for 10
min
Relative Centrifugal Force (RCF): - CORRECT ANSWERS-The force required to
separate 2 phases in a centrifuge.
Based upon 3 variables: Mass, speed, and radius
Expressed as #times greater than gravity
- CORRECT ANSWERS-RCF = 1.118 x 10-5 x r x rpm
Where:r = radius (cm) from center of rotation to bottom of tube in the rotor cavity
rpm = speed of rotation of the rotor in revolutions per minute
What is the RADIUS in a centrifuge? - CORRECT ANSWERS-
Horizontal-head - CORRECT ANSWERS-(swinging-bucket):
,bucket moves to allow tubes to spin in a horizontal position and rest in a vertical position
Particles travel constantly along the tube
Sediment is distributed evenly (flat surface)
WHMIS - CORRECT ANSWERS-- is a national hazard wide communication system
- Created to inform workers of the hazardous materials they work with
Why was WHMIS created? - CORRECT ANSWERS-- inform about the safety and
health hazards associated with the hazardous materials they might use at work
- Reduce injuries and illnesses associated with handling hazardous materials in the
workplace
GHS - CORRECT ANSWERS-- Global Harmonized System
-Move to standardize communication about hazardous products internationally
GHS consists of... - CORRECT ANSWERS-- Health hazards
- Physical hazards
- Environmental hazards (NOT ADOPTED BY CANADA)
WHMIS 2015 - CORRECT ANSWERS-- Canada's response to Globally Harmonized
System (GHS)
Consists of
- Labels (provides info about product and hazards)
- SDS (detailed info about hazards)
- Education (Training on how to safely handle hazardous products)
Suppliers - CORRECT ANSWERS-Classifying products
Establishing health and safety information
Labelling products
Preparing and providing SDS's
Employer - CORRECT ANSWERS-Ensure proper labels
Provide access to SDS's and update
Educate workers, and evaluate competency
, Train workers on how to handle, use, store, wear PPE for hazardous products, assess
competency
Provide control measures, adhere to Routine practices
Employees - CORRECT ANSWERS-Participating in education and training
Following prescribed safe work procedure
Identifying and controlling hazards
Hazardous Product - CORRECT ANSWERS-- Hazardous products are materials,
products, or substances that meet any of the criteria for one or more of the 32 GHS
Hazard Classes as defined in the Federal Hazardous Products Regulation
- Grouped into physical and health hazards
- WHMIS does not provide a comprehensive list of hazardous products, only a list of
hazard criteria.
Exclusions from WHMIS - CORRECT ANSWERS-- WHMIS may not apply if another act
or regulation is in place
- The following are exempt from the supplier responsibilities of WHMIS (no label or SDS
required):
•Consumer restricted products: products sold in stores that already have labels in
accordance with the Canada Consumer Product Safety Act
•Explosives: covered by the Explosives Act
•Cosmetics, drug, food, or devices: covered by the Food and Drugs Act
•Pesticides, herbicides, and insecticides: covered by the Pest Control Products Act
- Employer still responsible for for training employees on the safe use, handling, and
storage of these materials
more exceptions - CORRECT ANSWERS-Radioactive materials: covered by the
Nuclear Safety and Control Act
Wood and wood products•Manufactured articles that will not release chemicals
•Tobacco or tobacco products•In-house generated hazardous waste
With 100% Correct Answers / A + Grade.
Specifications (printed on the stem of the pipette): - CORRECT ANSWERS-Maximum
volume that can be transferred
Size of divisions on the pipette
What is the smallest volume that can be delivered accurately in this pipette? -
CORRECT ANSWERS-0.1 mL
Glass Pipettes - CORRECT ANSWERS-Volumetric (transfer) vs. Graduated
(measuring)
volumetric pipet - CORRECT ANSWERS-Calibrated to deliver a fixed volume of liquid
by drainage
Calibration Mark etched on stem, "bulb" in middle, gradual taper at tip
To be used when accuracy is required to deliver an exact amount
For ex: a 5 mL Volumetric pipette has a tolerance of 0.01 mL
Ostwald Pipette - CORRECT ANSWERS-similar to volumetric but with bulb closer to the
tip. Used for viscous fluids (ex. serum)
Graduated - CORRECT ANSWERS-Calibrated to deliver a volume of liquid between 2
calibration marks
an use to transfer various volumes
To be used when speed is required
Less accurate than volumetric pipettes
Ex: a 5 mL Graduated pipette has a tolerance of 0.02 mL
Two types of graduated pipettes - CORRECT ANSWERS-Mohr pipet - CORRECT
ANSWERS-Precipitate (pellet): the packed particles (solid) at the bottom of a
centrifuged tube
Supernatant: the liquid portion at the top of a centrifuged tube
,Decantation: separation of the supernatant from the pellet
- CORRECT ANSWERS-Rotor and carriers attached to the vertical shaft of a motor
and enclosed in a metal covering
Centrifuge: - CORRECT ANSWERS-apparatus used to separate substances of different
density or particle size, when suspended in a fluid, by spinning them about an axis in a
suitable container
Rotor and carriers attached to the vertical shaft of a motor and enclosed in a metal
covering
Rotor (centrifuge head): - CORRECT ANSWERS-Primary component of a centrifuge
which holds the material to be centrifuged and which is rotated by the drive system.
Centrifuges classified according to the following: - CORRECT ANSWERS-Benchtop vs.
floor
Refrigerated or not refrigerated
Rotor head type (fixed, hematocrit, swinging-bucket, angled)
Maximum speed attainable
Separating serum or plasma from blood cells
Normal separation of serum from clot: - CORRECT ANSWERS-Min of 1000 g for 10
min
Relative Centrifugal Force (RCF): - CORRECT ANSWERS-The force required to
separate 2 phases in a centrifuge.
Based upon 3 variables: Mass, speed, and radius
Expressed as #times greater than gravity
- CORRECT ANSWERS-RCF = 1.118 x 10-5 x r x rpm
Where:r = radius (cm) from center of rotation to bottom of tube in the rotor cavity
rpm = speed of rotation of the rotor in revolutions per minute
What is the RADIUS in a centrifuge? - CORRECT ANSWERS-
Horizontal-head - CORRECT ANSWERS-(swinging-bucket):
,bucket moves to allow tubes to spin in a horizontal position and rest in a vertical position
Particles travel constantly along the tube
Sediment is distributed evenly (flat surface)
WHMIS - CORRECT ANSWERS-- is a national hazard wide communication system
- Created to inform workers of the hazardous materials they work with
Why was WHMIS created? - CORRECT ANSWERS-- inform about the safety and
health hazards associated with the hazardous materials they might use at work
- Reduce injuries and illnesses associated with handling hazardous materials in the
workplace
GHS - CORRECT ANSWERS-- Global Harmonized System
-Move to standardize communication about hazardous products internationally
GHS consists of... - CORRECT ANSWERS-- Health hazards
- Physical hazards
- Environmental hazards (NOT ADOPTED BY CANADA)
WHMIS 2015 - CORRECT ANSWERS-- Canada's response to Globally Harmonized
System (GHS)
Consists of
- Labels (provides info about product and hazards)
- SDS (detailed info about hazards)
- Education (Training on how to safely handle hazardous products)
Suppliers - CORRECT ANSWERS-Classifying products
Establishing health and safety information
Labelling products
Preparing and providing SDS's
Employer - CORRECT ANSWERS-Ensure proper labels
Provide access to SDS's and update
Educate workers, and evaluate competency
, Train workers on how to handle, use, store, wear PPE for hazardous products, assess
competency
Provide control measures, adhere to Routine practices
Employees - CORRECT ANSWERS-Participating in education and training
Following prescribed safe work procedure
Identifying and controlling hazards
Hazardous Product - CORRECT ANSWERS-- Hazardous products are materials,
products, or substances that meet any of the criteria for one or more of the 32 GHS
Hazard Classes as defined in the Federal Hazardous Products Regulation
- Grouped into physical and health hazards
- WHMIS does not provide a comprehensive list of hazardous products, only a list of
hazard criteria.
Exclusions from WHMIS - CORRECT ANSWERS-- WHMIS may not apply if another act
or regulation is in place
- The following are exempt from the supplier responsibilities of WHMIS (no label or SDS
required):
•Consumer restricted products: products sold in stores that already have labels in
accordance with the Canada Consumer Product Safety Act
•Explosives: covered by the Explosives Act
•Cosmetics, drug, food, or devices: covered by the Food and Drugs Act
•Pesticides, herbicides, and insecticides: covered by the Pest Control Products Act
- Employer still responsible for for training employees on the safe use, handling, and
storage of these materials
more exceptions - CORRECT ANSWERS-Radioactive materials: covered by the
Nuclear Safety and Control Act
Wood and wood products•Manufactured articles that will not release chemicals
•Tobacco or tobacco products•In-house generated hazardous waste
