SOLUTIONS LATEST UPDATE
Reagants
1. Benedicts
2. Lugols
3. Grease Spot Test
4. Sudan IV
5. Ninhydrin
6. Biuret
Benedicts is used to test for
test for reducing sugars
Benedicts positive reaction
green
brick red
yellow
orange
Benedicts negative reaction
blue
Benedicts test used
7 test tubes of various solution in a test tube rack
a. water turned *blue*
,b. glucose turned *brown*
c. maltose turned *yellow*
d. albumin *turned: No change because protein*
e. starch turned *yellow*
f. milk turned *not change because of Lactose*
g. onion juice *not changed;*
**A TO D ARE WATER SOLUBLE*
Benedicts dependent variable
color liquid turns
Benedicts independent variable
type of solution
color
Benedicts Control
Water in beaker
Benedicts Constant Variables
1. amount of reagent
2. size of test tube
3. Heat
4. Same benedicts solution
what is unique about benedicts solution?
must be heated up in test tube inside of a beaker on a hot plate
Lugol's solution
, tests for starch
:Lugol's Positive reaction
blueish/black
Lugol's Negative reaction
brown
amber
soy sauce color
Lugol's formula
I2KI
Grease Spot test
tests for
1. fats
2. lipids
Grease Spot positive Reaction
grease spot stain on grease spot test paper
Grease Spot Negative Reaction
no grease spot
Grease Spot on:
brown paper
Sudan IV
tests for:
1. fats