7th Edition by Leventhal; Cheadle
Classify the protozoa?? - ANSWER:Sarcodina , e.histolytica, mastigophora, flagelletes
example giardia and leishmania, ciliophora example balantidum coli , sprozoa
plasmodium and cryptosporidium
What are hemo flagellates?? - ANSWER:Leishmania
Trypnosoma
What are apicomplexa - ANSWER:Sprozoa plasmodium and toxoplasma and
intestinal cryptosporidium and isospora
Which entameba are difficult to be distinghusihed?? - ANSWER:Histolytica, dispar,
moshkovskii
E.dispar is common in?? - ANSWER:Kenya
What test to use to differentaite look alike entameba - ANSWER:Pcr
Which panels can be used for e.histolytica - ANSWER:Biofore and luminex
What form of entameba we ingest?? - ANSWER:Cyst and they become trophozoite in
human intestine
Which stages of entameba passed in the feces - ANSWER:Both cyst and trophozoite
How many stool samples should be tested for histolytica and dispar - ANSWER:Three
What causes increase recovery of cyst in lab for histolytica and dispar -
ANSWER:Stool sample in formalin or other fixatives should be concentrated prior to
microscopic examination
For ameba species motility is seen in?? - ANSWER:Unstained saline
For ameba species trophozoite appearance and inclusions seen in - ANSWER:Saline,
formalin, methylene blue and permanant stain
For ameba species, trophozoite nucleus is seen in - ANSWER:Formalin, methylene
blue and permanant stain
For ameba species, nuclei of cyst is seen in?? - ANSWER:Formalin, iodine and
permanant stains
, For ameba species, chromatied bodies is seen in - ANSWER:Saline, formalin, iodine
and permanant stains
For ameba species, glycogen is seen in - ANSWER:Iodine and in permanat stain
What is the size of histolytica and dispar - ANSWER:12-15 micron
How stools are preserved if trophozoites have to be detected -
ANSWER:Merothiolate iodine formaldehyde or polyvinyl alcohol due to rapid
detoriation of trophozoites
How histolytica appear in trichrome as cyst and as trophozoite -
ANSWER:Trophozoite measure 15-20 micron, centrally located prominent
karyosome, psudopod while cyst measure 12-15 micron, chromatoid bodies with
blunt ends and have central vacuole
How E. histolytica is diagnosed?? - ANSWER:Enzyme immunoassays, EIA
Rapid immunochromatographis cartridge assay
What is ths requirement for EIA to diagnose histolytica - ANSWER:These tests require
the use of fresh or frozen stool specimen and cannot be used with preserved
specimen
What rapid immunochromatographic cartidge assay detects?? - ANSWER:Antigens of
histolytica and dispar but cannot differentiate them. Can also detect giardia and
cryptosporidium. Stool fresh or frozen
Treatment of amebiasis - ANSWER:Aymptomatic: with luminal agent, iodoquinol,
paromycin, diloxanide furoate. Symptomatic metronidazole followed by luminal
ageny
Name the non pathogenic ameba in GIT - ANSWER:Entameba coli
Entamoeba polecki
How trophozoite of enameba coli appears - ANSWER:Usually measure 15-50
microns, single nucleaus with eccentric karyosome and coarse irregulat peripheral
chromatin, cytoplam is coarsely granular and vaculated. Psudopodia may be see
How cyst of entameba coli appears?? - ANSWER:Usually spehrical but may be
elogated, measure 10-35 microns, mature cyst have 6 nuclei, karysome eccentric,
chromatin coarse n granular, diffuse glycogen, chromatid bodies if present has
splintered ends
What is the size of entameoba polecki - ANSWER:9 to 25 microns, there is single
nucleus measuring upto one third of the diameter of cyst