mip 302 practical Exam Questions with Verified
Answers
what is the primary stain in AF stains? - ✔✔carbon fuchsin
what would happen if you forgot the primary stain in an AF stain? - ✔✔AF + and -
cells would stain blue
what is the purpose of heating cells in AF staining? - ✔✔heat drives the primary stain
past the waxy cell wall
what is the purpose of the acid alcohol mix in AF staining? - ✔✔this decolonized
removes the primary stain form AF- bacteria because they have no mycolic acid in the
cell wall to retain the stain
what would happen if you forgot to decolorize in an AF stain? - ✔✔AF+ cells would
stain pink while AF- cells would stain purple
in the AF stain, what is the counterstain and its purpose? - ✔✔methylene blue turns
AF- cells blue and allows them to be visualized
what would hallen if you forgot to counterstain in an AF stain? - ✔✔AF+ cells would
be hot pink while AF- cells would be colorless
what does endospore staining differentiate between? - ✔✔endospore staining
differentiates between cells that can sporulate (form spores) and those who can't
what is the primary stain in an endospore stain? - ✔✔malachite green
, what is the purpose of heating cells in an endospore stain? - ✔✔to drive the primary
stain past the thick wall of the endospore
how do endospore + cells look under a microscope? - ✔✔green endospores within a
pink vegetative cells and/or free green endospores
what does a positive endospore stain indicate? - ✔✔a cell is able to produce
endospores in harsh environmental conditions
what does a negative endospore stain indicate? - ✔✔a cell is not able to produce
endospores
how do endospore - cells look under a microscope? - ✔✔pink vegetative cells
what is the purpose of decolorizer in an endospore stain? - ✔✔to remove the
primary stain from the vegetative bacterial cell, which does not retain the stain
what would happen if you forgot to decolorize in an endospore stain? -
✔✔endospores and vegetative cells would be green
what is the secondary stain in endospore staining and what is its purpose? -
✔✔safranin turns vegetative and endospore - cells pink/red and allows them to be
visualized
what are cocci? - ✔✔round shaped bacteria
What are diplococci? - ✔✔pairs of cocci
Answers
what is the primary stain in AF stains? - ✔✔carbon fuchsin
what would happen if you forgot the primary stain in an AF stain? - ✔✔AF + and -
cells would stain blue
what is the purpose of heating cells in AF staining? - ✔✔heat drives the primary stain
past the waxy cell wall
what is the purpose of the acid alcohol mix in AF staining? - ✔✔this decolonized
removes the primary stain form AF- bacteria because they have no mycolic acid in the
cell wall to retain the stain
what would happen if you forgot to decolorize in an AF stain? - ✔✔AF+ cells would
stain pink while AF- cells would stain purple
in the AF stain, what is the counterstain and its purpose? - ✔✔methylene blue turns
AF- cells blue and allows them to be visualized
what would hallen if you forgot to counterstain in an AF stain? - ✔✔AF+ cells would
be hot pink while AF- cells would be colorless
what does endospore staining differentiate between? - ✔✔endospore staining
differentiates between cells that can sporulate (form spores) and those who can't
what is the primary stain in an endospore stain? - ✔✔malachite green
, what is the purpose of heating cells in an endospore stain? - ✔✔to drive the primary
stain past the thick wall of the endospore
how do endospore + cells look under a microscope? - ✔✔green endospores within a
pink vegetative cells and/or free green endospores
what does a positive endospore stain indicate? - ✔✔a cell is able to produce
endospores in harsh environmental conditions
what does a negative endospore stain indicate? - ✔✔a cell is not able to produce
endospores
how do endospore - cells look under a microscope? - ✔✔pink vegetative cells
what is the purpose of decolorizer in an endospore stain? - ✔✔to remove the
primary stain from the vegetative bacterial cell, which does not retain the stain
what would happen if you forgot to decolorize in an endospore stain? -
✔✔endospores and vegetative cells would be green
what is the secondary stain in endospore staining and what is its purpose? -
✔✔safranin turns vegetative and endospore - cells pink/red and allows them to be
visualized
what are cocci? - ✔✔round shaped bacteria
What are diplococci? - ✔✔pairs of cocci
