MIP 302 Exam Prep Questions with Verified
Answers
when preparing a slide for staining why is it important to only put a small amount on the
slide? - ✔✔if you have too many you won't be able to really see them, and they
won't stain as well
why are slides allowed to dry completely before heat fixing? - ✔✔if there is water on
it while you heat fix it, it causes cell lysis
How does one determine if ones cells are algae of protozoans? - ✔✔the algae were
green/yellow color
the protozoan were moving and colorless
what is the function of protozoan in rumen fluid? - ✔✔digest cellulose
how to calculate total magnification? - ✔✔objective magnification x ocular
magnification
Resolving power? - ✔✔resolving power (RP) = [(.5) (wavelength)] / numerical
aperture (NA)
which resolving power is better? - ✔✔smaller resolving power is better
oil immersion? - ✔✔gets rid of air, prevents light from bending away from the
microscope lens
, difference between tryptic soy agar and tryptic soy broth? - ✔✔broth is a liquid, agar
is a solid
broth is used as a growth medium
agar is used as isolation medium
what type of medium is tryptic soy? - ✔✔an all-purpose medium-- supports growth
of bacteria that do not have special nutritional needs
What is turbidity? - ✔✔when the broth becomes cloudy, and bacteria has grown
why are broths and slants better suited as culture mediums as opposed to isolation
mediums? - ✔✔broth is a liquid so the bacteria would be floating in it
a slant is not used bc the bacteria are too close together to be isolated
why are agar plates well-suited or bacteria isolation? - ✔✔bc you are able to dilute it,
gradually getting more and more diluted till they are isolated
how do you know isolation had been achieved on your plate? - ✔✔you see individual
colonies
colony shapes? - ✔✔circular
irregular
Answers
when preparing a slide for staining why is it important to only put a small amount on the
slide? - ✔✔if you have too many you won't be able to really see them, and they
won't stain as well
why are slides allowed to dry completely before heat fixing? - ✔✔if there is water on
it while you heat fix it, it causes cell lysis
How does one determine if ones cells are algae of protozoans? - ✔✔the algae were
green/yellow color
the protozoan were moving and colorless
what is the function of protozoan in rumen fluid? - ✔✔digest cellulose
how to calculate total magnification? - ✔✔objective magnification x ocular
magnification
Resolving power? - ✔✔resolving power (RP) = [(.5) (wavelength)] / numerical
aperture (NA)
which resolving power is better? - ✔✔smaller resolving power is better
oil immersion? - ✔✔gets rid of air, prevents light from bending away from the
microscope lens
, difference between tryptic soy agar and tryptic soy broth? - ✔✔broth is a liquid, agar
is a solid
broth is used as a growth medium
agar is used as isolation medium
what type of medium is tryptic soy? - ✔✔an all-purpose medium-- supports growth
of bacteria that do not have special nutritional needs
What is turbidity? - ✔✔when the broth becomes cloudy, and bacteria has grown
why are broths and slants better suited as culture mediums as opposed to isolation
mediums? - ✔✔broth is a liquid so the bacteria would be floating in it
a slant is not used bc the bacteria are too close together to be isolated
why are agar plates well-suited or bacteria isolation? - ✔✔bc you are able to dilute it,
gradually getting more and more diluted till they are isolated
how do you know isolation had been achieved on your plate? - ✔✔you see individual
colonies
colony shapes? - ✔✔circular
irregular
