Pathogenic Microbiology Test 4
Questions And Verified Correct Answers.
What are some characteristics of the Enterobacteriaceae? - Answer all members are gram negative
bacilli or coccobacilli
all members are cytochrome oxidase negative
all members grow on MacConkey agar
Do all enterobacteriaceace members ferment lactose? - Answer No
Swarming colonies on sheep blood agar would lead one to suspect: - Answer Proteus
What organisms produce H2S or gas? - Answer Salmonella
Proteus
Citrobacter
Does Shigella produce H2S or gas? - Answer No
The production of deaminase is found in which of the following groups of bacteria? - Answer Proteus,
Providencia, and Morganella
The most frequently implicated species of Shigella in the United States is: - Answer S. sonnei
Enterohemorrhagic E. coli (EHEC) is: - Answer Also known as verocytoxic E. coli
May be associated with hemolytic uremic syndrome
May often serotype as 0157:H7
Is the H antigen found in the cell wall? - Answer No
,On Hektoen Enteric agar, Shigella appears as: - Answer Clear or colorless colonies.
Are members of the tripe Klebsielleae VP positive or negative? - Answer Positive
Is Proteus mirabilis indole positive or negative? - Answer Negative
In the ____________ __________ pathway for glucose fermentation, acetoin is detected. - Answer
Voges Proskauer
Rapid urease activity is characteristic of the genus _________. - Answer Proteus
___________ ___________ causes plague, which is transmitted through fleas and rodents. - Answer
Yersinia pestis
__________ ____________ produces DNAse, lipase, gelatinase and sometimes a red pigment. - Answer
Serratia marcescens
________ media is used to isolate Yersinia enterocolitica. - Answer CIN
Cefsulodin-irgasin-novobiocin
A surgical wound specimen produces pink colonies on MacConkey agar. There is no growth on CNA and
abundant flat, spreading gray mucoid colonies on blood agar.
The next four questions are talking about this case study above.
This specimen contains: - Answer A gram negative bacillus that ferments lactose
The TSI is yellow over yellow with cracks but no blackening. This would be interpreted as: - Answer
Ferments glucose with gas production, ferments lactose and/ or sucrose
, The IMVC reactions are - - + + which would eliminate __________ as a possible organism. - Answer E.
coli
The organism is nonmotile, LDC +, ODC -, ADH - which would most likely indicate that the isolate is: -
Answer Klebsiella pneumoniae
129) Infection of the urinary tract is most frequently associated with:
a. Staphylococcus aureus
b. Escherichia coli
c. Enterococcus faecalis
d. Serratia marcescens - Answer b (>80% of uncomplicated UTIs are caused by E coli.)
130) MacConkey media for screening suspected cases of hemorrhagic E coli O157:H7 must contain:
a. indole
b. citrate
c. sorbitol
d. lactose - Answer c (Sorbitol replaces lactose in MacConkey. E coli 0157:H7 does not ferment sorbitol,
whereas other species of E coli are positive for fermentation of sorbitol. This makes the media a good
screen for 0157:H7.)
131) Members of the family Enterobacteriaceae share which one of the following characteristics?
a. produce cytochrome oxidase
b. ferment lactose
c. produce beta-hemolysis
d. reduce nitrate to nitrite - Answer d (Members of Enterobacteriaceae are oxidase negative, ferment
glucose, and reduce nitrate to nitrite.)
132) Which one of the following genera is among the least biochemically reactive members of the
Enterobacteriaceae?
a. Proteus
b. Pseudomonas
Questions And Verified Correct Answers.
What are some characteristics of the Enterobacteriaceae? - Answer all members are gram negative
bacilli or coccobacilli
all members are cytochrome oxidase negative
all members grow on MacConkey agar
Do all enterobacteriaceace members ferment lactose? - Answer No
Swarming colonies on sheep blood agar would lead one to suspect: - Answer Proteus
What organisms produce H2S or gas? - Answer Salmonella
Proteus
Citrobacter
Does Shigella produce H2S or gas? - Answer No
The production of deaminase is found in which of the following groups of bacteria? - Answer Proteus,
Providencia, and Morganella
The most frequently implicated species of Shigella in the United States is: - Answer S. sonnei
Enterohemorrhagic E. coli (EHEC) is: - Answer Also known as verocytoxic E. coli
May be associated with hemolytic uremic syndrome
May often serotype as 0157:H7
Is the H antigen found in the cell wall? - Answer No
,On Hektoen Enteric agar, Shigella appears as: - Answer Clear or colorless colonies.
Are members of the tripe Klebsielleae VP positive or negative? - Answer Positive
Is Proteus mirabilis indole positive or negative? - Answer Negative
In the ____________ __________ pathway for glucose fermentation, acetoin is detected. - Answer
Voges Proskauer
Rapid urease activity is characteristic of the genus _________. - Answer Proteus
___________ ___________ causes plague, which is transmitted through fleas and rodents. - Answer
Yersinia pestis
__________ ____________ produces DNAse, lipase, gelatinase and sometimes a red pigment. - Answer
Serratia marcescens
________ media is used to isolate Yersinia enterocolitica. - Answer CIN
Cefsulodin-irgasin-novobiocin
A surgical wound specimen produces pink colonies on MacConkey agar. There is no growth on CNA and
abundant flat, spreading gray mucoid colonies on blood agar.
The next four questions are talking about this case study above.
This specimen contains: - Answer A gram negative bacillus that ferments lactose
The TSI is yellow over yellow with cracks but no blackening. This would be interpreted as: - Answer
Ferments glucose with gas production, ferments lactose and/ or sucrose
, The IMVC reactions are - - + + which would eliminate __________ as a possible organism. - Answer E.
coli
The organism is nonmotile, LDC +, ODC -, ADH - which would most likely indicate that the isolate is: -
Answer Klebsiella pneumoniae
129) Infection of the urinary tract is most frequently associated with:
a. Staphylococcus aureus
b. Escherichia coli
c. Enterococcus faecalis
d. Serratia marcescens - Answer b (>80% of uncomplicated UTIs are caused by E coli.)
130) MacConkey media for screening suspected cases of hemorrhagic E coli O157:H7 must contain:
a. indole
b. citrate
c. sorbitol
d. lactose - Answer c (Sorbitol replaces lactose in MacConkey. E coli 0157:H7 does not ferment sorbitol,
whereas other species of E coli are positive for fermentation of sorbitol. This makes the media a good
screen for 0157:H7.)
131) Members of the family Enterobacteriaceae share which one of the following characteristics?
a. produce cytochrome oxidase
b. ferment lactose
c. produce beta-hemolysis
d. reduce nitrate to nitrite - Answer d (Members of Enterobacteriaceae are oxidase negative, ferment
glucose, and reduce nitrate to nitrite.)
132) Which one of the following genera is among the least biochemically reactive members of the
Enterobacteriaceae?
a. Proteus
b. Pseudomonas
