BIOCHEM ACS STUDY GUIDE EXAM
QUESTIONS AND ANSWERS 2024
BESTZONE
BIOCHEM
8/29/24
,BIOCHEM ACS STUDY GUIDE EXAM
Each of the following subcellular structures contains DNA EXCEPT
A. the nucleus.
B. ribosomes.
C. chloroplasts.
D. mitochondria. - B
In plant and animal cells, the three-dimensional integrity of the cell
is maintained, in part, by an internal network of filaments called
A. the cell wall.
B. the Golgi apparatus.
C. the cytoskeleton.
D. the endoplasmic reticulum. - C
Of the following, which are prokaryotes?
A. viruses
B. bacteria
C. yeast
D. plant cells - B
G° for the conversion of glycerol-3-phosphate to glycerol + Pi is -2.2 kcal/mole. Thus,
you can conclude that
A. this reaction will occur spontaneously.
B. this reaction may or may not occur spontaneously, depending on the
actual concentrations of reactants and products.
C. this reaction will only occur spontaneously if it is coupled to
another reaction that has a more favorable G°.
D. this reaction is so strongly favorable that it could be coupled to
drive an otherwise unfavorable cell reaction. - B
BIOCHEM ACS STUDY GUIDE EXAM
,BIOCHEM ACS STUDY GUIDE EXAM
In most biochemical reactions, ∆PV is
A. negligible
B. positive
C. negative
D. dependent on ∆E - A
According to the Second Law of Thermodymnamics,
A. the total entropy change in any reaction that occurs spontaneously must be greater
than 0.
B. the total entropy change in any reaction that occurs spontaneously must be less
than 0.
C. the total entropy of the universe remains constant.
D. the total entropy of the universe is always decreasing. - A
Which of the following is true?
A. Liquid and solid water have the same number of hydrogen bonds per molecule of
water
B. Liquid water has more hydrogen bonds per molecule than does solid water
C. Solid water has more hydrogen bonds per molecule than does liquid water
D. Hydrogen bonding is a major factor in the structure of gaseous water
E. None is true - C
What is the pH of an aqueous solution that has a [OH-] of 3.45 x 10-5 M?
A. 3.47
B. 4.47
C. 5.53
D. 9.54
E. none of the above - D
BIOCHEM ACS STUDY GUIDE EXAM
, BIOCHEM ACS STUDY GUIDE EXAM
A peptide was found to have a molecular mass of about 650 and upon hydrolysis
produced Ala, Cys, Lys, Phe, and Val in a 1:1:1:1:1 ratio. The peptide upon treatment
with Sanger's reagent produced DNP-Cys and exposure to carboxypeptidase produced
valine. Chymotrypsin treatment of the peptide produced a dipeptide that contained
sulfur and has a UV absorbance, and a tripeptide. Exposure of the peptide to trypsin
produced a dipeptide and a tripeptide. Deduce the sequence of the peptide.
A. Val-Ala-Lys-Phe-Cys
B. Cys-Lys-Phe-Ala-Val
C. Cys-Ala-Lys-Phe-Val
D. Cys-Phe-Lys-Ala-Val
E. Val-Phe-Lys-Ala-Cys - D
Which amino acid has an R-group pKa closest to physiological pH?
A. lysine
B. histidine
C. glutamate
D. arginine
E. aspartate - B
Which is true regarding the orientation of the R-groups in beta structures?
A. In the parallel beta sheet structures the amino acid R-groups are all on the same
side of the sheet.
B. In the antiparallel beta sheet structure the R-groups on alternate strands are on the
same side of the sheet.
C. In parallel and antiparallel sheets consecutive R-groups on each peptide strand
alternate sides of the sheet.
D. Because of the free rotation around the alpha carbon, the R-groups can seek the
least crowded region and are not restricted to specific side of the sheet.
E. a and b - C
A reagent commonly employed to cleave disulfide bonds in proteins is
BIOCHEM ACS STUDY GUIDE EXAM
QUESTIONS AND ANSWERS 2024
BESTZONE
BIOCHEM
8/29/24
,BIOCHEM ACS STUDY GUIDE EXAM
Each of the following subcellular structures contains DNA EXCEPT
A. the nucleus.
B. ribosomes.
C. chloroplasts.
D. mitochondria. - B
In plant and animal cells, the three-dimensional integrity of the cell
is maintained, in part, by an internal network of filaments called
A. the cell wall.
B. the Golgi apparatus.
C. the cytoskeleton.
D. the endoplasmic reticulum. - C
Of the following, which are prokaryotes?
A. viruses
B. bacteria
C. yeast
D. plant cells - B
G° for the conversion of glycerol-3-phosphate to glycerol + Pi is -2.2 kcal/mole. Thus,
you can conclude that
A. this reaction will occur spontaneously.
B. this reaction may or may not occur spontaneously, depending on the
actual concentrations of reactants and products.
C. this reaction will only occur spontaneously if it is coupled to
another reaction that has a more favorable G°.
D. this reaction is so strongly favorable that it could be coupled to
drive an otherwise unfavorable cell reaction. - B
BIOCHEM ACS STUDY GUIDE EXAM
,BIOCHEM ACS STUDY GUIDE EXAM
In most biochemical reactions, ∆PV is
A. negligible
B. positive
C. negative
D. dependent on ∆E - A
According to the Second Law of Thermodymnamics,
A. the total entropy change in any reaction that occurs spontaneously must be greater
than 0.
B. the total entropy change in any reaction that occurs spontaneously must be less
than 0.
C. the total entropy of the universe remains constant.
D. the total entropy of the universe is always decreasing. - A
Which of the following is true?
A. Liquid and solid water have the same number of hydrogen bonds per molecule of
water
B. Liquid water has more hydrogen bonds per molecule than does solid water
C. Solid water has more hydrogen bonds per molecule than does liquid water
D. Hydrogen bonding is a major factor in the structure of gaseous water
E. None is true - C
What is the pH of an aqueous solution that has a [OH-] of 3.45 x 10-5 M?
A. 3.47
B. 4.47
C. 5.53
D. 9.54
E. none of the above - D
BIOCHEM ACS STUDY GUIDE EXAM
, BIOCHEM ACS STUDY GUIDE EXAM
A peptide was found to have a molecular mass of about 650 and upon hydrolysis
produced Ala, Cys, Lys, Phe, and Val in a 1:1:1:1:1 ratio. The peptide upon treatment
with Sanger's reagent produced DNP-Cys and exposure to carboxypeptidase produced
valine. Chymotrypsin treatment of the peptide produced a dipeptide that contained
sulfur and has a UV absorbance, and a tripeptide. Exposure of the peptide to trypsin
produced a dipeptide and a tripeptide. Deduce the sequence of the peptide.
A. Val-Ala-Lys-Phe-Cys
B. Cys-Lys-Phe-Ala-Val
C. Cys-Ala-Lys-Phe-Val
D. Cys-Phe-Lys-Ala-Val
E. Val-Phe-Lys-Ala-Cys - D
Which amino acid has an R-group pKa closest to physiological pH?
A. lysine
B. histidine
C. glutamate
D. arginine
E. aspartate - B
Which is true regarding the orientation of the R-groups in beta structures?
A. In the parallel beta sheet structures the amino acid R-groups are all on the same
side of the sheet.
B. In the antiparallel beta sheet structure the R-groups on alternate strands are on the
same side of the sheet.
C. In parallel and antiparallel sheets consecutive R-groups on each peptide strand
alternate sides of the sheet.
D. Because of the free rotation around the alpha carbon, the R-groups can seek the
least crowded region and are not restricted to specific side of the sheet.
E. a and b - C
A reagent commonly employed to cleave disulfide bonds in proteins is
BIOCHEM ACS STUDY GUIDE EXAM
