BIO-120L pH and Buffer Lab

Chemistry of Life: pH and Buffers



Katie Letson



7/10/2022

, 1



Data

Activity 1

Calculate the volume of 1 M NaOH needed to prepare 12.0 mL of a 0.10 M
solution.
MiVi=MfVf
Vi=MfVf/Mi
Vi=0.1*12/1 Vi=1.2 mL
1.2 mL of `1M NaOH is needed to prepare the solution.


Calculate the volume of 1 M HCl needed to prepare 12.0 mL of a 0.10 M
solution.
MiVi=MfVf
Vi=MfVf/Mi
Vi=0.1 *12/`1 Vi=1.2 mL
1.2 mL of 1M HCI is needed to prepare the solution.




Data Table 1

pH after 1 pH after 10 pH after 1 pH after 10
Initial pH drop 0.1M drops 0.1M drop 0.1M drops 0.1M
HCl HCl NaOH NaOH
≤ pH 6 ≤ pH 4 ≤ pH 4 ≥ pH 10 ≥ pH 10
Water

pH 4 ≤ pH 4 ≤ pH 4 ≤ pH 4 ≤ pH 5 ≤ pH 6
buffer
≤ pH 6
pH 6 ≤ pH 6 ≤ pH 6 ≤ pH 5 pH 6.5
buffer

pH 8 ≤ pH 8 ≤ pH 7 ≤ pH 7 ≤ pH 8 ≤ pH 9
buffer



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