Micro 210 Lab
Exam 1 Questions
and Complete
Solutions Graded
A+
Denning [Date] [Course title]
, Explain why oil is required for the proper use of the 100X objective on a bright light microscope. -
Answer: The light passing through would be refracted by its passage from air into glass and glass into air,
so most of the light would not appear. Oil has the same refractive index as glass, so the light won't be
minimized.
Differences between eukaryotes and prokaryotes - Answer: Eukaryotes have membrane bound
organelles and nucleus; prokaryotes do not. Eukaryotes are larger. Eukaryotic cell walls are composed of
polysaccharides and prokaryotic cell walls are composed of peptidoglycan. (refer to list for more)
Saprobe - Answer: something that derives nourishment from nonliving or decaying organic matter (Fungi
are an example)
What is the main atmospheric molecule consumed by algae? Released? - Answer: Consumed:carbon
dioxide
Released: oxygen
What is an agar slant and what is the utensil used to inoculate it? - Answer: solidified growth medium in
a test tube, allowed to solidify at an angle; inoculation loop
What is agar deep and what is used to inoculate it? - Answer: solidified medium in a test tube, allowed
to solidify while upright; inoculation loop or needle
What is agar pour? - Answer: liquefied medium poured into large test tube and intended for pouring
into a Petri plate later
What is agar plate? - Answer: liquefied medium poured into a Petri plate in order to create a solid
surface of growing bacteria
inoculation - Answer: microorganism are transferred from one medium to another
simple stain - Answer: use of a single basic dye; highlights the entire microorganism to visualize cell
shapes and structures
Exam 1 Questions
and Complete
Solutions Graded
A+
Denning [Date] [Course title]
, Explain why oil is required for the proper use of the 100X objective on a bright light microscope. -
Answer: The light passing through would be refracted by its passage from air into glass and glass into air,
so most of the light would not appear. Oil has the same refractive index as glass, so the light won't be
minimized.
Differences between eukaryotes and prokaryotes - Answer: Eukaryotes have membrane bound
organelles and nucleus; prokaryotes do not. Eukaryotes are larger. Eukaryotic cell walls are composed of
polysaccharides and prokaryotic cell walls are composed of peptidoglycan. (refer to list for more)
Saprobe - Answer: something that derives nourishment from nonliving or decaying organic matter (Fungi
are an example)
What is the main atmospheric molecule consumed by algae? Released? - Answer: Consumed:carbon
dioxide
Released: oxygen
What is an agar slant and what is the utensil used to inoculate it? - Answer: solidified growth medium in
a test tube, allowed to solidify at an angle; inoculation loop
What is agar deep and what is used to inoculate it? - Answer: solidified medium in a test tube, allowed
to solidify while upright; inoculation loop or needle
What is agar pour? - Answer: liquefied medium poured into large test tube and intended for pouring
into a Petri plate later
What is agar plate? - Answer: liquefied medium poured into a Petri plate in order to create a solid
surface of growing bacteria
inoculation - Answer: microorganism are transferred from one medium to another
simple stain - Answer: use of a single basic dye; highlights the entire microorganism to visualize cell
shapes and structures
