Unit 2 Microbiology
Lab Exam
Questions and
Complete Solutions
Graded A+
Denning [Date] [Course title]
, Recombination is referring to combining two sources of: - Answer: dna
The enzyme which cuts apart DNA is called: - Answer: restriction endonuclease
In the simulation that you performed at home, how did you simulate the enzyme which cuts apart DNA?
- Answer: scissors
The enzyme which was used to cut the DNA was used to cut: - Answer: the human insulin gene out of
the human genome
open the plasmid
Could a different enzyme be used to cut out the human gene than was used to open up the plasmid? -
Answer: no
The restriction enzyme you used was called EcoRI. What does Eco stand for? - Answer: Escherichia coli
What did the tape simulate? - Answer: The bonding between the ends of the plasmid to the ends of the
human insulin gene
an action of the enzyme called ligase
The circular white strip of paper represented a: - Answer: bacterial plasmid
The blue strip of paper represented the: - Answer: human insulin gene
T/F To be able to connect the ends of the plasmid to the ends of the human insulin gene, you needed to
use a restriction endonuclease which made a staggered cut along the double stranded DNA. This would
allow the single stranded ends to overlap. Moreover, the base pairing would have to follow the rule of A
with T and G with C. - Answer: true
What medium do you use for a throat culture? - Answer: blood agar
Lab Exam
Questions and
Complete Solutions
Graded A+
Denning [Date] [Course title]
, Recombination is referring to combining two sources of: - Answer: dna
The enzyme which cuts apart DNA is called: - Answer: restriction endonuclease
In the simulation that you performed at home, how did you simulate the enzyme which cuts apart DNA?
- Answer: scissors
The enzyme which was used to cut the DNA was used to cut: - Answer: the human insulin gene out of
the human genome
open the plasmid
Could a different enzyme be used to cut out the human gene than was used to open up the plasmid? -
Answer: no
The restriction enzyme you used was called EcoRI. What does Eco stand for? - Answer: Escherichia coli
What did the tape simulate? - Answer: The bonding between the ends of the plasmid to the ends of the
human insulin gene
an action of the enzyme called ligase
The circular white strip of paper represented a: - Answer: bacterial plasmid
The blue strip of paper represented the: - Answer: human insulin gene
T/F To be able to connect the ends of the plasmid to the ends of the human insulin gene, you needed to
use a restriction endonuclease which made a staggered cut along the double stranded DNA. This would
allow the single stranded ends to overlap. Moreover, the base pairing would have to follow the rule of A
with T and G with C. - Answer: true
What medium do you use for a throat culture? - Answer: blood agar
