ASCP(MB) Exam Questions and Complete Solutions Graded A+

ASCP(MB) Exam
Questions and
Complete Solutions
Graded A+
Denning [Date] [Course title]

, FRET - Answer: (Fluorescent Resonance Energy Transfer)

Uses TWO distinct probes to generate a signal.

probe 1: DONOR 5'

probe 2: REPORTER 3'

both anneal onto one strand of amplicon: SIGNAL.



When does a SYBR green probe generate a signal during PCR? - Answer: Extension



When does a MBG probe generate a signal during PCR? - Answer: (minor groove binder) Annealing



When does a molecular beacon probe generate a signal during PCR? - Answer: Annealing



When does a TaqMan probe generate a signal during PCR? - Answer: Extension (chomp chomp)



When does a Scorpion probe generate a signal during PCR? - Answer: Extension



Which technique employs the amplification of the signal resulting from probe:target hybridization rather
than by amplifying the target or the probe? - Answer: branched DNA analysis (BDA)

It's performed by capturing a target nucleic acid onto a microtiter plate and then adding an amplifier
probe to greatly increase the signal resulting from the captured nucleic acid.



SIGNAL AMPLIFICATION TECHNIQUES - Answer: Branched DNA analysis

Hybrid Capture Assays

Cleavage-Based Amplification

Cycling Probes



Branched DNA analysis - Answer: SIGNAL AMPLIFICATION

Capture probes immobilize target, extender probes bind to target, amplifiers give off loads of signal.