GM3 PRACTICE FINAL EXAM|UPDATED WITH RATIONALE Q&A GRADED A+

GM3 PRACTICE FINAL EXAM|UPDATED
WITH RATIONALE Q&A GRADED A+
The field of study within genetics that focuses on the study of genomes is called? -
Genomics

The project that was initiated with the intention of sequencing and analyzing the human
genome as well as the genomes of several organisms was called the what project? -
Human Genome

The entire complement of information contained within the DNA sequence of an
organism is known as the? - Genome

Restriction enzymes are enzymes that can
a). create site-directed mutans
b). cute the DNA backbone and reveal protein-binding sequences
c). cut the DNA backbone at specific sequences
d). identify host cells containing vectors - c). cut the DNA backbone at specific
sequences

What is a restriction fragment?
a). a protein domain within a restriction enzyme
b). a DNA sequence that can be recognized by a restriction enzyme
c). a gene that encodes a restriction enzyme
d). a fragment of DNA generated by a restriction enzyme - d). a fragment of DNA
generated by a restriction enzyme

The act of cutting a piece of DNA with a restriction enzyme is called - Digestion

"Sticky ends" created by cutting DNA with a restriction enzyme are
a) single-stranded overhangs of DNA
b) sequences that confer antibiotic resistance
c). sequences that can be used to initiate replication of a DNA molecule - a). single-
stranded overhangs of DNA

The technique that is used to separate charged molecules based on their movement in
an electric field is called? - Electrophoresis

During electrophoresis, which of the following DNA fragments will travel the greatest
distance from the well?
a). a DNA fragment with a high GC content
b). a DNA fragment with a low GC content
c). a large DNA fragment
d). a small DNA fragment - d). a small DNA fragment

, During electrophoresis, the rate at which different linear DNA molecules will move
through a gel depends on differences in their
a). charge densities
b). gene content
c). sizes
d). GC content - c). sizes

The process of isolating a single fragment of DNA from a complex mixture and making
many exact copies of it is called molecular - cloning

A carrier DNA molecule that allows the DNA fragment of interest to be transported into a
host cell, replicated, and then purified is called a(n) - Vector

A group of replicated DNA molecules that are exact replicas of one another is called a
DNA - clone

A researcher may use restriction enzymes to digest the DNA of an organism. The
fragments of DNA are then recombined into many vectors. The collection of recombinant
vectors is called a(n) - library

A recombinant DNA molecule has covalently linked fragments from
a). genes that encode proteins and pseudogenes
b). a vector and an inserted fragment
c). bacteria and viruses
d). a bacterial gene and a human gene - b). a vector and an inserted fragment

A small circular DNA molecule that is often used as a vector in gene cloning is called
a(n) - plasmid

A vector requires an origin or replication so that it can be
a). copied many times by the host cell
b). altered by the host cell
c). analyzed through DNA sequencing
d). used to express proteins - a). copied many times by the host cell

The process by which a cell or organism takes up a foreign DNA molecule is called -
transformation

A genomic library is a collection of cellular clones containing copies of
a). each full-length chromosomes from an organism
b). all DNA sequences in the genome of an organism
c). only the coding sequences of genes from an organism - b). all DNA sequences in the
genome of an organism

The enzyme that catalyzes DNA replication is called - DNA polymerase