ASCP Blood Bank Practice Questions with Correct
Answers
Results of a serum sample tested against a panel of reagent red cells
gives presumptive evidence of an alloantibody directed against a high
incidence antigen. Further investigation to confirm the specificity should
include which of the following?
A.serum testing against red cells from random donors
B.serum testing against red cells known to lack high incidence antigens
C.serum testing against enzyme treated autologous red cells
D.testing of an eluate prepared from the patient's red cells Correct
Answer-B. serum testing against red cells known to lack high incidence
antigens
Fetal neonatal alloimmune thrombocytopenia (FNAIT) is caused by
which of the following maternal antibodies?
A.IgM alloantibodies against ABO antigens
B.IgG alloantibodies against HLA antigens
C.IgM alloantibodies against glycoprotein V (GPV) antigens
D.IgG alloantibodies against HPA antigens Correct Answer-D. IgG
alloantibodies against HPA antigens
FNAIT is due to maternal IgG alloantibodies crossing the placenta and
reacting with fetal platelet HPA antigens.
,The purpose of testing with anti-A,B is to detect:
A.anti-A1
B.anti-A2
C.subgroups of A
D.subgroups of O Correct Answer-Subgroups of A
Ax cells react more strongly with anti-A,B than with anti-A. If anti-A is
nonreactive, Ax cells may be detected with anti-A,B.
What increment of platelets in the typical 70-kg human is expected to
result from each single unit of Platelets transfused to a non-HLA-
sensitized recipient?
A.3,000 - 5,000/µL
B.5,000 - 10,000/µL
C.20,000 - 25,000/µL
D.25,000 - 30,000/µL Correct Answer-B.5,000 - 10,000/µL
One unit of Platelets made from whole blood should cause the platelet
count to increase from 5,000-10,000/µL.
All donor blood testing must include:
A.complete Rh phenotyping
B.anti-CMV testing
C.direct antiglobulin test
,D.serological test for syphilis Correct Answer-D.serological test for
syphilis
A sample gives the following results:
Cells with Anti A: 3+ Anti B: 4+
Serum with A1: 2+ and B: 0
Which lectin should be used first to resolve this discrepancy?
A.Ulex europaeus
B.Arachis hypogaea
C.Dolichos biflorus
D.Vicia graminea Correct Answer-C.Dolichos biflorus
Dolichos biflorus plant seed extract forms complexes with N-
acetylgalactosamine. When properly diluted, it can distinguish between
A1 donor cells and all other subgroups of A.
Transfusion-associated HTLV I/II incidence is low due to the following
laboratory testing:
A.nucleic acid testing for HTLV I/II
B.HTLV I core antibody testing by ELISA
C.HTLV II surface antigen testing by chemiluminescent immunoassay
(ChLIA)
, D.IgG antibody testing for HTLV I/II by ELISA or ChLIA Correct
Answer-D.IgG antibody testing for HTLV I/II by ELISA or ChLIA
A patient demonstrates 4+ reactivity with all red cells tested and the
autocontrol is nonreactive. This high incidence antibody was suspected
to be related to the P1PK blood group system as the patient is the rare p
phenotype. What antibody specificity should be suspected?
A.anti-IP1
B.anti-P2
C.anti-PP1Pk
D.anti-P1 Correct Answer-C.anti-PP1Pk
Anti-PP1Pk is the high incidence antibody made by individuals with the
p phenotype. This antibody has an association with early spontaneous
abortion.
Which unit should be selected for exchange transfusion if the newborn is
group A, Rh-positive and the mother is group A, Rh-positive with anti-
c?
A.A, CDe/CDe
B.A, cDE/cDE
C.O, cde/cde
D.A, cde/cde Correct Answer-A.A, CDe/CDe
In suspected cases of hemolytic disease of the fetus and newborn, what
significant information can be obtained from the baby's blood smear?
Answers
Results of a serum sample tested against a panel of reagent red cells
gives presumptive evidence of an alloantibody directed against a high
incidence antigen. Further investigation to confirm the specificity should
include which of the following?
A.serum testing against red cells from random donors
B.serum testing against red cells known to lack high incidence antigens
C.serum testing against enzyme treated autologous red cells
D.testing of an eluate prepared from the patient's red cells Correct
Answer-B. serum testing against red cells known to lack high incidence
antigens
Fetal neonatal alloimmune thrombocytopenia (FNAIT) is caused by
which of the following maternal antibodies?
A.IgM alloantibodies against ABO antigens
B.IgG alloantibodies against HLA antigens
C.IgM alloantibodies against glycoprotein V (GPV) antigens
D.IgG alloantibodies against HPA antigens Correct Answer-D. IgG
alloantibodies against HPA antigens
FNAIT is due to maternal IgG alloantibodies crossing the placenta and
reacting with fetal platelet HPA antigens.
,The purpose of testing with anti-A,B is to detect:
A.anti-A1
B.anti-A2
C.subgroups of A
D.subgroups of O Correct Answer-Subgroups of A
Ax cells react more strongly with anti-A,B than with anti-A. If anti-A is
nonreactive, Ax cells may be detected with anti-A,B.
What increment of platelets in the typical 70-kg human is expected to
result from each single unit of Platelets transfused to a non-HLA-
sensitized recipient?
A.3,000 - 5,000/µL
B.5,000 - 10,000/µL
C.20,000 - 25,000/µL
D.25,000 - 30,000/µL Correct Answer-B.5,000 - 10,000/µL
One unit of Platelets made from whole blood should cause the platelet
count to increase from 5,000-10,000/µL.
All donor blood testing must include:
A.complete Rh phenotyping
B.anti-CMV testing
C.direct antiglobulin test
,D.serological test for syphilis Correct Answer-D.serological test for
syphilis
A sample gives the following results:
Cells with Anti A: 3+ Anti B: 4+
Serum with A1: 2+ and B: 0
Which lectin should be used first to resolve this discrepancy?
A.Ulex europaeus
B.Arachis hypogaea
C.Dolichos biflorus
D.Vicia graminea Correct Answer-C.Dolichos biflorus
Dolichos biflorus plant seed extract forms complexes with N-
acetylgalactosamine. When properly diluted, it can distinguish between
A1 donor cells and all other subgroups of A.
Transfusion-associated HTLV I/II incidence is low due to the following
laboratory testing:
A.nucleic acid testing for HTLV I/II
B.HTLV I core antibody testing by ELISA
C.HTLV II surface antigen testing by chemiluminescent immunoassay
(ChLIA)
, D.IgG antibody testing for HTLV I/II by ELISA or ChLIA Correct
Answer-D.IgG antibody testing for HTLV I/II by ELISA or ChLIA
A patient demonstrates 4+ reactivity with all red cells tested and the
autocontrol is nonreactive. This high incidence antibody was suspected
to be related to the P1PK blood group system as the patient is the rare p
phenotype. What antibody specificity should be suspected?
A.anti-IP1
B.anti-P2
C.anti-PP1Pk
D.anti-P1 Correct Answer-C.anti-PP1Pk
Anti-PP1Pk is the high incidence antibody made by individuals with the
p phenotype. This antibody has an association with early spontaneous
abortion.
Which unit should be selected for exchange transfusion if the newborn is
group A, Rh-positive and the mother is group A, Rh-positive with anti-
c?
A.A, CDe/CDe
B.A, cDE/cDE
C.O, cde/cde
D.A, cde/cde Correct Answer-A.A, CDe/CDe
In suspected cases of hemolytic disease of the fetus and newborn, what
significant information can be obtained from the baby's blood smear?
