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Gel Electrophoresis Questions & Verified Answers

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Gel Electrophoresis Questions & Verified Answers

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Gel Electrophoresis Questions & Verified Answers

What is the purpose of gel electrophoresis? Right Ans - To separate DNA
fragments based on their size using electricity

What does "phoresis" mean? Right Ans - To transmit (move location,
migrate)

What three gel ingredients are required for gel electrophoresis? Right Ans -
Agarose, buffer, and distilled H2O

What is agarose? Right Ans - A sugar found in seaweed

What is a characteristic of agarose? Right Ans - It is gelatin-like

What purpose does agarose serve in gel electrophoresis? Right Ans - It is
the matrix (obstacle course) for DNA fragments (it separates them by size)

What is the buffer? Right Ans - A salt solution

What purpose does the buffer serve in gel electrophoresis? Right Ans - It
maintains pH (at 7 which is neutral) and conducts electricity which allows the
DNA fragments to migrate through the agarose gel

What purpose does the distilled H2O serve in gel electrophoresis? Right
Ans - It is the solvent

Why does the H2O need to be distilled? Right Ans - It contains no chlorine
or other contaminants that could damage DNA

How is the agarose power measured? Right Ans - On an electronic balance,
after zeroing out the weighing tray

How is the distilled H2O measured? Right Ans - In a graduated cylinder
(measured to the meniscus)
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