Gel Electrophoresis – Questions With Detailed Answers

Gel Electrophoresis – Questions With Detailed Answers

What is gel electrophoresis? Right Ans - A way to sort and measure DNA
strands

Why is gel electrophoresis necessary? (size) Right Ans - DNA is so small,
most microscopes can't see it

What charge is in the gel where the strands are injected? Right Ans -
Negative

What charge is in the gel opposite of where the gel is injected? Right Ans -
Positive

Why must an electrical charge be applied to the gel? Right Ans - So the DNA
can move

Which sized DNA strands show up farthest from the gel walls? Right Ans -
Short

How are the bands on the gel formed? Right Ans - During electrophoresis,
the strands move and make sorted groups

What must be done to the gel in order to see the bands on the gel? Right
Ans - The gel must be stained

What materials are needed to make a gel? Right Ans - Powdered agarose,
buffer, flask, microwave, gel mold, and comb

What is agarose? Right Ans - Dried powder similar to gelatin but made
from seaweed

What is the buffer? Right Ans - Salt water

What is the buffer used for? Right Ans - It will let the electrical charges flow
through the gel