MB(ASCP) PRACTICE EXAM WITH CORRECT
180+ QUESTIONS WITH CORRECTRY
ANALYZED ANSWERS (ACTUAL EXAM)
ALREADY GRADED A+ LATEST 2024 – 2025
What is the best way to store RNA for longer than a month?
-In nuclease free (DEPC-treated) water, at -20°C or -80°C
-In TrizolⓇ, at -20°C or -80°C
-In Ethanol, at -20°C or -80°C
-In DMSO, at -20°C or -80°C - ANSWERS-In Ethanol, at -20°C or -80°C
How many hydrogen bonds are formed between one C:G base pair?
1
2
3
4 - ANSWERS-3
,Next Generation Sequencing uses: - ANSWERS-Short sequence reads
Purines and pyrimidines differ from each other in that: - ANSWERS-Purines have
two rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - ANSWERS-Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - ANSWERS-1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not
depend on which of the following factors?
Net charge of the molecule
Size of the molecule
,Shape of the molecule
Nucleotide sequence of the molecule - ANSWERS-Nucleotide sequence of the
molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - ANSWERS-Initiation,
exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - ANSWERS-3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit.
The physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for
mutation screening in the Huntington gene. Testing with standard PCR indicates
, that patient has Huntington Disease, HD. Which of the following would be
consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - ANSWERS-85 CAG repeats in the
Huntington gene
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - ANSWERS-Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - ANSWERS-
Thymine
What genes would be screened in a breast cancer panel? - ANSWERS-HER2,
ERBB2, BRCA1
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
180+ QUESTIONS WITH CORRECTRY
ANALYZED ANSWERS (ACTUAL EXAM)
ALREADY GRADED A+ LATEST 2024 – 2025
What is the best way to store RNA for longer than a month?
-In nuclease free (DEPC-treated) water, at -20°C or -80°C
-In TrizolⓇ, at -20°C or -80°C
-In Ethanol, at -20°C or -80°C
-In DMSO, at -20°C or -80°C - ANSWERS-In Ethanol, at -20°C or -80°C
How many hydrogen bonds are formed between one C:G base pair?
1
2
3
4 - ANSWERS-3
,Next Generation Sequencing uses: - ANSWERS-Short sequence reads
Purines and pyrimidines differ from each other in that: - ANSWERS-Purines have
two rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - ANSWERS-Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - ANSWERS-1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not
depend on which of the following factors?
Net charge of the molecule
Size of the molecule
,Shape of the molecule
Nucleotide sequence of the molecule - ANSWERS-Nucleotide sequence of the
molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - ANSWERS-Initiation,
exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - ANSWERS-3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit.
The physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for
mutation screening in the Huntington gene. Testing with standard PCR indicates
, that patient has Huntington Disease, HD. Which of the following would be
consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - ANSWERS-85 CAG repeats in the
Huntington gene
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - ANSWERS-Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - ANSWERS-
Thymine
What genes would be screened in a breast cancer panel? - ANSWERS-HER2,
ERBB2, BRCA1
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
