BIO 340 Genetics ASU
Module 6 Exam
Questions and Complete
Solutions Graded A+
Denning [Date] [Course title]
, polymerase chain reaction (PCR) - Correct Ans: One of the analytical methods used to visualize the DNA
replication process; gives insight to deciphering DNA variation. (lab-facilitated version of DNA
replication)
Gel electrophoresis - Correct Ans: a technique commonly used to separate biological molecules based
on size and biochemical characteristics, such as charge and polarity.
pulsed-field gel electrophoresis (PFGE) - Correct Ans: To separate very large DNA fragments, such as
chromosomes or viral genomes, agarose gel electrophoresis can be modified by periodically alternating
the orientation of the electric field during ______________________
ethidium bromide (intercalating agent) - Correct Ans: locations of the DNA or RNA fragments in the gel
can be detected by adding _________________, that inserts into the nucleic acids at non-specific
locations and can be visualized when exposed to ultraviolet light.
chain termination method - Correct Ans: method involves DNA replication of a single-stranded template
with the use of a primer and a regular deoxynucleotide (dNTP).
ddNTPs - Correct Ans: monomers that are missing a hydroxyl group (-OH) at the site at which another
nucleotide usually attaches to form a chain.
next generation sequencing - Correct Ans: a group of automated techniques used for rapid DNA
sequencing; can generate sequences of hundreds of thousands or millions of short fragments (25 to 600
base pairs) in one day.
pyrosequencing - Correct Ans: DNA sequencing technology that is based on the generation and
detection of a pyrophosphate group liberated from a nucleotide triphosphate
pyrophosphate (PPi) - Correct Ans: The ester dimer of phosphate; released when a new nucleotide is
joined to a growing strand of DNA by DNA polymerase
probes - Correct Ans: Short DNA fragments called _____________ are designed and labeled with
radioactive or fluorescent dyes to aid detection for the presence of certain DNA sequences.
Module 6 Exam
Questions and Complete
Solutions Graded A+
Denning [Date] [Course title]
, polymerase chain reaction (PCR) - Correct Ans: One of the analytical methods used to visualize the DNA
replication process; gives insight to deciphering DNA variation. (lab-facilitated version of DNA
replication)
Gel electrophoresis - Correct Ans: a technique commonly used to separate biological molecules based
on size and biochemical characteristics, such as charge and polarity.
pulsed-field gel electrophoresis (PFGE) - Correct Ans: To separate very large DNA fragments, such as
chromosomes or viral genomes, agarose gel electrophoresis can be modified by periodically alternating
the orientation of the electric field during ______________________
ethidium bromide (intercalating agent) - Correct Ans: locations of the DNA or RNA fragments in the gel
can be detected by adding _________________, that inserts into the nucleic acids at non-specific
locations and can be visualized when exposed to ultraviolet light.
chain termination method - Correct Ans: method involves DNA replication of a single-stranded template
with the use of a primer and a regular deoxynucleotide (dNTP).
ddNTPs - Correct Ans: monomers that are missing a hydroxyl group (-OH) at the site at which another
nucleotide usually attaches to form a chain.
next generation sequencing - Correct Ans: a group of automated techniques used for rapid DNA
sequencing; can generate sequences of hundreds of thousands or millions of short fragments (25 to 600
base pairs) in one day.
pyrosequencing - Correct Ans: DNA sequencing technology that is based on the generation and
detection of a pyrophosphate group liberated from a nucleotide triphosphate
pyrophosphate (PPi) - Correct Ans: The ester dimer of phosphate; released when a new nucleotide is
joined to a growing strand of DNA by DNA polymerase
probes - Correct Ans: Short DNA fragments called _____________ are designed and labeled with
radioactive or fluorescent dyes to aid detection for the presence of certain DNA sequences.
