Histology/Cytology Questions and Answers with complete solutions

Histology/Cytology Questions and Answers with complete solutions a good decalcifying agent should do this - ANS- remove the minerals within 2 - 4 days an important step in tissue processing - ANS- fixation, it prevents cell morphology changes and degradation autopsy - ANS- the removal of tissues from a dead body bluing stained tissue means - ANS- making the pH alkaline cell nucleoplasm contains - ANS- DNA cytological smears are fixed with - ANS- 95% alcohol direct smears for cytology are made from this usually - ANS- sputum fixative for routine hematology smear stain - ANS- methanol gross dissection - ANS- during this calcium is found to be present in tissue histology cassette - ANS- used to hold tissue during processing microtome - ANS- a piece of equipment used in Histology which is not temperature dependent microtome - ANS- the instrument used for cutting paraffin embedded tissue most commonly used decalcification fluid - ANS- HNO3 most important step in regressive hematoxylin staining - ANS- differentiation in acid alcohol pap smear detects this - ANS- various cancers procedure for gross dissection - ANS- decalcification after fixation reason for embedding tissue in parraffin wax - ANS- so the tissue may be cut into sections smears from body fluids for cytology exams are fixed with - ANS- 95% alcohol synovial fluid is obtained by - ANS- needle aspiration of a joint the acid alcohol used in differentiate hematoxylin stained smears - ANS- 1% HCI in 70% alcohol the black mark on a tissue is positioned - ANS- up the mucus from the lungs, bronchi and trachea use this to test for malignant cells - ANS- sputum the preparation done to slides in histology - ANS- tissue slide preparations submitted for microscopic examination are cut at 4 to 6 microns the process of dehydration is - ANS- utilizing a series of ascending grads of alcohol the routine processing of tissue utilizes - ANS- 12 different stations the third stain in pap stain - ANS- EA50 the volume of fixative to tissue in specimen jars - ANS- ten times the volume of the tissue this appears when the tissue has been embedded - ANS- a black mark this can be used to remove calcium from tissue - ANS- EDTA reagent this happens if the tissues are not completely dehydrated when they go into Xylol - ANS- they go cloudy this happens if water gets into the molten wax - ANS- it produces crystals this is for specimens that are larger than 5mm for processing - ANS- mega-cassette this is not tested in the cytology department - ANS- liver tissue this is not used to preserve tissue - ANS- formic acid this is performed immediately after clearing - ANS- wax infiltration this is poisonous - ANS- bouin's fluid this is used in the process of clearing - ANS- xylene is used in histology as a clearing agent this is used to differentiate - ANS- acid alcohol