MSP Week 3 Exam Questions & Answers 100% Correct

how was the event of fusion captured - ANSWER-by flash freezing of the stimulated NMJ and electron microscopy what is the issue with electron microscopy - ANSWER-only a snapshot, making it difficult to see fusion mechanism ____ mediate all fusion events in the cell - ANSWER-SNARE proteins ____ use the same mechanism to enter cells - ANSWER-viral spike proteins true or false: SNAP25 provides 2 alpha helices so only 3 proteins exist in the SNARE complex, but other fusion reactions can have 2 proteins replacing SNAP25 - ANSWER-true what SNARE is located in the center of the CORE complex and forms the pore - ANSWER-R-SNARE how is viral fusion different from SV fusion - ANSWER-to charge, a protease needs to cleave a piece of the SPIKE protein/viral fusion protein to activate it and then can be released and anchored into the membrane, SPIKE protein rearranges to wrap into the 4 helices to initiate fusion how is viral fusion similar to SV fusion - ANSWER-different processes BUT the energy of the helices wrapped around each other is conserved, viruses have to carry everything by themselves (all SNAREs "with" them) docking - ANSWER-Munc18 opens syntaxin's SNARE motif (N-terminus) so it can bind to SNAP25