MLS 2211 Unit 1 Chem WSU with Verified Solutions

MLS 2211 Unit 1 Chem WSU with Verified Solutions Dilution The amount of sample divided by the total amount of solution You have 1 mL of sample and 4 mL of H2O - How many parts do you have? - Whats the ratio? - What is the dilution factor? - What is the concentration of the sample after its diluted? - (4+1) = 5 parts - 1:5 - The dultion factor is 5 - 0.20 The dilution factor is the deminator 5 You have 5 mL of sample and 5 mL of H2O - how many parts do you have? - Whats the ratio? - What is the dilution factor? - What is the concentration of the sample after its diluted? - 10 parts - 1:2 - 2 - 0.5 You have 5 mL of sample and 10 mL of H2O - How many parts do you have? - Whats the ratio - What is the dilution factor? - What is the concentration of the sample after its been diluted? - 15 parts - 1:3 - 3 - 0.3333 serial dilution A stepwise dilution of a substance in solution When would we use serial dilution in the lab? when we want to access antibody concentration/strength aka titers. How would you prepare a 1:24 dilution? - 1 part sample - 23 part H2O - add the component to an actual amount ex sample has 5 uL and the H2O has 115uL - total volume = 120uL -120/5 = 24 therefore 1:24 What if you have too much of something? dilution Dilution (The golden clinical chemistry equation) C1V1=C2V2 c1 = v1= - initial concentration - initial volume c2= v2= - concentration of the diluted solution - volume of the diluted solution 1 mL of a 50 mg/dL sample is diluted up to a final volume of 15 mL. What is the final concentration? 3.333 mg/dl 7 mL of a 23 mg/dL sample is dilutes up to a final volume of 83 ml. what is the final concentration? 1.94 mg/dl 1 mL of a 20 mg/dL sample is diluted and the final concentration