ACS Biochemistry Exam Questions and
Correct Solutions 2024
Describe the structural changes in hemoglobin that allow for cooperative binding of O2 - Answer -
Binding of O2 in one subunit causes a conformational change in an adjacent subunit allowing O2 to bind
Which amino acids in "a" would be charged at pH 7? - Answer -Asp, Glu, Arg, His, Lys
Which amino acids are negatively charged? - Answer -Asp, Glu
which amino acids are positively charged? - Answer -Arg, His, Lys
How are beta sheets stabilized in proteins? - Answer -Hydrogen bonding between peptide backbone
groups
Does myoglobin show cooperativity in O2 binding? If not, what structural differences account for this? -
Answer -Myoglobin does not show cooperativity because it is monomeric
What is a transition state inhibitor (how does its structure compare to the enzyme stubstrate?) how does
its affinity for the enzyme compare to the enzyme's substrate? - Answer -Similar to the substrate; binds
to the active site with greater affinity than the substrate
would it be advantageous for these potential drugs to covalently bind to the enzyme? why or why not? -
Answer -Not advantageous because the urea cycle would shut down
From a practical standpoint, would it be advantageous for these potential drugs to be less chemically
stable than the substrate? - Answer -No, because of shelf life and time to reach target
In DNA, which bases hydrogen bond? - Answer -A-T; G-C
In each base pair, how many hydrogen bonds are there? - Answer -A-T=2; G-C=3
Which base-pair would require a higher temperature to destroy the hydrogen bonds? - Answer -G-C
Based on the mechanism of alkaline hydrolysis of RNA, why is DNA less susceptible to alkaline
hydrolysis? - Answer -DNA lacks the 2' hydroxyl group
What is the difference between a reducing and nonreducing sugar? - Answer -Reducing sugars have a
free anomeric carbon that in the linear form can reduce an oxidizing agent.
what is a reducing monosaccharide? - Answer -fructose or glucose
what is a reducing disaccharide? - Answer -lactose
Correct Solutions 2024
Describe the structural changes in hemoglobin that allow for cooperative binding of O2 - Answer -
Binding of O2 in one subunit causes a conformational change in an adjacent subunit allowing O2 to bind
Which amino acids in "a" would be charged at pH 7? - Answer -Asp, Glu, Arg, His, Lys
Which amino acids are negatively charged? - Answer -Asp, Glu
which amino acids are positively charged? - Answer -Arg, His, Lys
How are beta sheets stabilized in proteins? - Answer -Hydrogen bonding between peptide backbone
groups
Does myoglobin show cooperativity in O2 binding? If not, what structural differences account for this? -
Answer -Myoglobin does not show cooperativity because it is monomeric
What is a transition state inhibitor (how does its structure compare to the enzyme stubstrate?) how does
its affinity for the enzyme compare to the enzyme's substrate? - Answer -Similar to the substrate; binds
to the active site with greater affinity than the substrate
would it be advantageous for these potential drugs to covalently bind to the enzyme? why or why not? -
Answer -Not advantageous because the urea cycle would shut down
From a practical standpoint, would it be advantageous for these potential drugs to be less chemically
stable than the substrate? - Answer -No, because of shelf life and time to reach target
In DNA, which bases hydrogen bond? - Answer -A-T; G-C
In each base pair, how many hydrogen bonds are there? - Answer -A-T=2; G-C=3
Which base-pair would require a higher temperature to destroy the hydrogen bonds? - Answer -G-C
Based on the mechanism of alkaline hydrolysis of RNA, why is DNA less susceptible to alkaline
hydrolysis? - Answer -DNA lacks the 2' hydroxyl group
What is the difference between a reducing and nonreducing sugar? - Answer -Reducing sugars have a
free anomeric carbon that in the linear form can reduce an oxidizing agent.
what is a reducing monosaccharide? - Answer -fructose or glucose
what is a reducing disaccharide? - Answer -lactose
