CRISPR-Cas9 Final Practice Exam Questions 2024.

CRISPR-Cas9 Final Practice Exam Questions 2024.What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kinds of research that previously would have been too expensive or required too much time to carry out How many types of CRISPR mechanisms been identified? - CORRECT ANSWER 3, but type II is the most studiedd What does CRISPR stand for? - CORRECT ANSWER Clusters of Regularly Interspaced Short Palindromic Repeats What is the CRISPR system comprised of? - CORRECT ANSWER A small piece of RNA and a protein called Cas9 What does the RNA in the CRISPR system do? - CORRECT ANSWER Guides Cas9 to particular part of a gene What does Cas9 in the CRISPR system do? - CORRECT ANSWER Breaks both strands of DNA at the specific spot creating a double-stranded break (DSB) Where does the DSB occur? - CORRECT ANSWER 3bp upstream from PAM in the genome