SBB Review
Band 3 - correct answer macrocomplex of proteins including:
-Rh
-RhAG
-LW (ICAM-4)
-GPA
-GPB
-CD47
ATP source for RBCs - correct answer 90% from Glycolysis (Embden-Meyerhoff pathway)
10% from pentose phosphate pathway
Methemoglobin Reductase Pathway - correct answer necessary to maintain heme iron in ferrous (Fe2+)
state
Deficiency causes accumulation of methemoglobin, nonfunctional from of Hgb
Leubering Rapaport Shunt - correct answer permits accumulation of 2,3-DPG
"Tense form" of Hgb - correct answer DEOXYGENATED
widening of beta-chain
lower affinity for O2
expels 2,3-DPG
"Relaxed form" of Hgb - correct answer OXYGENATED
beta-chain pulled together
higher affinity for O2
expels 2,3-DPG
,"Shift Right" - correct answer higher P50 value
decreased pH (increased H+ ion), acidosis
increased 2,3-DPG
higher temperature
high pCO2
Seen in compensated anemia
"Shift Left" - correct answer lower P50 value
increased pH (decresed H+ ion), alkalosis
decreasede 2,3-DPG
lower temeratures
low pCO2
Seen in storage of RBC
Type 2 precursor - correct answer Gal-GlcNAc
B1-4
Type 1 precursor - correct answer Gal-GlcNAc
B1-3
Secreted (Se, Le)
Type 2 H antigen transeferase & Sugar - correct answer FUT-1
Alpha-2-L-fucosyltransferase
,Fuctose alpha1-2
A antigen transferase & sugar - correct answer D-galactosaminetrasferase
N-acetylgalactosamine alpha1-3
B antigen transferase & sugar - correct answer D-galacotsyltransferase
galactose alpha1-3
Ax subgroup - correct answer weakly positive anti-A
weakly positive anti-A,B
Am subgroup - correct answer Negative anti-A
Secretes A & H
Ael subgroup - correct answer Negative anti-A
Secretes H only
Highest expression of H
Lowest expression of H - correct answer O>A2>B>A2B>A1>A1B> H deficient
Lewis transferase & Sugar - correct answer FUT-3
Fuctose alpha1-4
Type 1 H antigen transferase & sugar - correct answer FUT-2
Fuctose alpha1-2
, anti-Le bh vs anti-Le bl - correct answer Anti-Le bh= reacts best with Leb & H are on rbc
reacts best with group O and A2
does not react with A1 & B
Anti-Le bl= reacts independently of amount of H on rbc
best antisera to use when typing RBC
autoanti-H, -HI is most common in... - correct answer A1 patients, have the least amount of H
Elevated i antigen associated with... - correct answer cord cells
reticulocytes in megaloblastic anemia
leukemia
stressed erythropoiesis
I gene - correct answer IGnT
adds GlcNAc to Gal
starts branching/ multivalent
anti-i associated with what disease - correct answer infectious mono
autoanti-i, -I commonly seen in patients with.... - correct answer Mycoplasma pneumoniae infections
Lymphoproliferative disorder (Waldenstrom macroglobinemia)
P1K & P2K lack what gene/ antigen - correct answer B3GalNAct1
missing P antigen (also Gb5 & LKE)
p lack what gene/ antigen - correct answer A4GALT
Band 3 - correct answer macrocomplex of proteins including:
-Rh
-RhAG
-LW (ICAM-4)
-GPA
-GPB
-CD47
ATP source for RBCs - correct answer 90% from Glycolysis (Embden-Meyerhoff pathway)
10% from pentose phosphate pathway
Methemoglobin Reductase Pathway - correct answer necessary to maintain heme iron in ferrous (Fe2+)
state
Deficiency causes accumulation of methemoglobin, nonfunctional from of Hgb
Leubering Rapaport Shunt - correct answer permits accumulation of 2,3-DPG
"Tense form" of Hgb - correct answer DEOXYGENATED
widening of beta-chain
lower affinity for O2
expels 2,3-DPG
"Relaxed form" of Hgb - correct answer OXYGENATED
beta-chain pulled together
higher affinity for O2
expels 2,3-DPG
,"Shift Right" - correct answer higher P50 value
decreased pH (increased H+ ion), acidosis
increased 2,3-DPG
higher temperature
high pCO2
Seen in compensated anemia
"Shift Left" - correct answer lower P50 value
increased pH (decresed H+ ion), alkalosis
decreasede 2,3-DPG
lower temeratures
low pCO2
Seen in storage of RBC
Type 2 precursor - correct answer Gal-GlcNAc
B1-4
Type 1 precursor - correct answer Gal-GlcNAc
B1-3
Secreted (Se, Le)
Type 2 H antigen transeferase & Sugar - correct answer FUT-1
Alpha-2-L-fucosyltransferase
,Fuctose alpha1-2
A antigen transferase & sugar - correct answer D-galactosaminetrasferase
N-acetylgalactosamine alpha1-3
B antigen transferase & sugar - correct answer D-galacotsyltransferase
galactose alpha1-3
Ax subgroup - correct answer weakly positive anti-A
weakly positive anti-A,B
Am subgroup - correct answer Negative anti-A
Secretes A & H
Ael subgroup - correct answer Negative anti-A
Secretes H only
Highest expression of H
Lowest expression of H - correct answer O>A2>B>A2B>A1>A1B> H deficient
Lewis transferase & Sugar - correct answer FUT-3
Fuctose alpha1-4
Type 1 H antigen transferase & sugar - correct answer FUT-2
Fuctose alpha1-2
, anti-Le bh vs anti-Le bl - correct answer Anti-Le bh= reacts best with Leb & H are on rbc
reacts best with group O and A2
does not react with A1 & B
Anti-Le bl= reacts independently of amount of H on rbc
best antisera to use when typing RBC
autoanti-H, -HI is most common in... - correct answer A1 patients, have the least amount of H
Elevated i antigen associated with... - correct answer cord cells
reticulocytes in megaloblastic anemia
leukemia
stressed erythropoiesis
I gene - correct answer IGnT
adds GlcNAc to Gal
starts branching/ multivalent
anti-i associated with what disease - correct answer infectious mono
autoanti-i, -I commonly seen in patients with.... - correct answer Mycoplasma pneumoniae infections
Lymphoproliferative disorder (Waldenstrom macroglobinemia)
P1K & P2K lack what gene/ antigen - correct answer B3GalNAct1
missing P antigen (also Gb5 & LKE)
p lack what gene/ antigen - correct answer A4GALT
