MB (ASCP) Practice Exam 2024 100% Correct

MB (ASCP) Practice Exam 2024 100% Correct What does barcoding do in Next Generation Sequencing? - Answer ️️ -Adds unique tag to all nucleic acids within one sample so that various samples can be mixed What is the best way to store RNA for longer than a month? - Answer ️️ -In Ethanol, at -20°C or -80°C Testing for Aldehyde Dehydrogenase deficiency is important in patients with: - Answer ️️ - Alcohol dependence During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an incoming nucleotide? - Answer ️️ -Beta The 5' capping process creates what type of linkage? - Answer ️️ -5'-5' Branched DNA amplification (bDNA) is best classified as: - Answer ️️ -Signal amplification Which PCR method has the highest specificity? - Answer ️️ -Nested PCR This polymerase is involved in "replicates mitochondrial DNA": - Answer ️️ -Pol γ What factors can affect nucleic acid hybridization? - Answer ️️ -1.) G:C ratio of bases 2.) pH of reaction 3.) temp of reaction What test methods can be used to test for translocations? - Answer ️️ -1.) FISH 2.) Southern Blot 3.) Reverse-transcriptase PCR Mantle cell lymphoma (MCL) is caused by what translocation? - Answer ️️ -t(11;14) mecA gene product creates resistance to which drug? - Answer ️️ -Penicillin What are some characteristics of eukaryotic DNA transcription? - Answer ️️ -1.) Multiple RNA polymerases 2.) TATA-binding proteins 3.) Transcription factors This restriction enzyme "digests and removes a methyl group from Adenine": - Answer ️️ - Type I What enzyme is involved in LCR? - Answer ️️ -DNA Ligase HCV type 4 responds poorly to what type of treatment? - Answer ️️ -Ribavarin/Interferon A CSF sample for hematologic evaluation should be tested within how many hours of collection? - Answer ️️ -1 hour What genes are tested for patients with breast cancer? - Answer ️️ -BCRA 1 & 2 and HOXB13 What are pre-analytical variables that can have a negative impact on clinical testing? - Answer ️️ -1.) Storage conditions 2.) Patient identifiers 3.) Anticoagulant in collection tubes 4.) Transport procedures What type of restriction enzyme has methylation activity? - Answer ️️ -Type I Trisomy 21 is... - Answer ️️ -Down syndrome What is the best testing method to detect the Leiden mutation? - Answer ️️ -PCR-RFLP What translocation is associated with Burkitt's lymphoma? - Answer ️️ -t(8;14) What are the steps of PCR? - Answer ️️ -denaturing, annealing, extension (elongation) What does the term consanguinity refer to? - Answer ️️ -Individuals that share a common ancestor or are considered "of the same blood" What are sources of DNA damage? - Answer ️️ -1.) Chemical damage 2.) Attack by water 3.) Radiation damage Mutation in UGT1A1 affects metabolism of what drug? - Answer ️️ -Sorafenib List 3 examples of trinucleotide repeat disorders. - Answer ️️ -1.) Spinocerebellar Ataxia Type 8 2.) Huntington Disease 3.) Fragile X How many log10 reductions in HIV viral load is considered to be successful upon treatment? - Answer ️️ -0.5log10