Molecular Diagnostics Final (Exam + Quiz MC) /verified/

Molecular Diagnostics Final (Exam + Quiz MC) /verified/ d (primers) *Ans* *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) *Ans* *Complementarity of primer pairs at the 3' ends will result in what artifacts?* a. Primer dimers b. Large mis-primed products c. No amplification d. 5' end degradation of the primers d (secondary structure) *Ans* *A DNA sequence with high GC content is more likely to have what property that interferes with PCR amplification?* a. Tendency to degrade b. Enzyme inactivation c. Lower Tm d. Secondary structure b (nested PCR offers increased specificity and yield of product) *Ans* *What is the advantage of a nested PCR procedure?* a. Nested PCR is less labor-intensive. b. Nested PCR offers increased specificity and yield of product. c. Nested PCR has a shorter reaction time than standard PCR. d. Nested PCR is less expensive than standard PCR. c (real time PCR is quantitative) *Ans* *Real-time PCR differs from standard PCR in which way?* a. Standard PCR is faster. b. Real-time PCR is less sensitive. c. Real-time PCR is quantitative. d. Standard PCR is more specific. d (PCR products from a previous reaction) *Ans* *Which of the following is the most likely source of PCR contamination?* a. Unfiltered dust particles b. Environmental fungi c. Eyelashes d. PCR products from a previous reaction d (hybrid capture assay) *Ans* *In which of the following nucleic acid amplification procedures is a hybrid nucleic acid labeled with detectable signal?*