WGU BioChem OA Review with Complete and Verified Solutions

Males and Females are equally affected Autosomal The process of copying DNA in the lab. Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers stick to the DNA that you want to copy and ADD DNA Polymerase What is Annealing? The Polymerase Chain Reaction is heated and DNA Polymerase adds nucleotides to build a new DNA strand. What is elongating?Ligase What is NOT involved in PCR? It is used to repair damage to bases caused by harmful molecules by removing the base that is damaged and replacing it. #1 GLYCOSYLASES see the damaged DNA. #2 DNA Polymerase puts the right one back #3 DNA Ligase seals it. What is base excision repair (BER)? DNA Glycolysase sees the damage and removes it, DNA polymerase puts the tight base back, DNA ligase seals it back up. What are the 3 steps in base excision repair? 1 How many nucleotides does base excision repair remove? Mismatch Repair (MMR). It is the only one to occur during REPLICATION - DURING THE PROOFREADING What is it called when a large section of the nucleotide is removed from the DNA so that DNA polymerase can try again? (This corrects DNA damage) Mismatch Repair (MMR) repairs errors such as G with T instead of G with C. DNA Polymerase fixes it. This happens during replication and MMR removes a large section of the nucleotide from the DNA and DNA Polymerase tries again. What repairs a base mismatch? When DNA polymerase takes an individual nucleotide and matches them to the parental sequences to ensure a correct pair. (it must bind with RNA primer to work). ie: DNA Polymerase binds to DNA to make RNA What is DNA Transcription?