CMMB Tutorial 2 Test questions and answers graded A+ 2024/2025

CMMB Tutorial 2 Test Discovered hh gene? - correct answer CNV and EW Discovered a homolog to Dhh in mice? - correct answer Andrew Mcmahon Discovered Shh in Chickens - correct answer Cliff Tobin Discovered the Zebrafish Homolog - correct answer Phil Ingram A region in the posterior margin of the limb bud that contains retinoic acid that induces Shh - correct answer Zone polarizing activity (ZPA) Mirror duplicate of digits. - correct answer What happens when you implant ZPA into anterior region of limb bud? Protein that acts in a concentration dependent manner - correct answer Morphogen 1. Vitamin A derivative 2. Too much exposure leads to birth defects 3.Normal level outside of posterior but not enough to induce ZPA formation - correct answer Retinoic Acid Use Homologs from similar organisms - correct answer How to identify vertebrate ZPA? Compared Desert Hedgehog to Drosophila hedgehog ***Most conserved sequence between 161-237aa*** - correct answer Riddle et al Methods to identify Shh - correct answer Degenerate PCR Chichen limb cDNA library sequencing