Microbiology Lab Midterm (Lab Notebook Questions) with Complete Solutions

Microbiology Lab Midterm (Lab Notebook Questions) with Complete Solutions What is located in the headpiece that bends light rays toward the ocular lenses? - Answer-prism Name the only type of paper and three fluids that may be used to clean objective and ocular lenses? -Answer--lens paper -acetone -xylene -ethyl alcohol What is the most important aspect in caring for the microscope? -Answer-keeping it clean and using it properly Which way does the stage move when you rotate the coarse adjustment knob towards you? -Answer-down Make a statement concerning the distance the stage moves up or down when using the coarse adjustment compound to the fine adjustment. -Answer-Fine adjustment: sharpens image Coarse adjustment: moves the stage and provides the most focus by changing distance between lens and specimen Your microscope is equipped with a series of 3 lenses and is therefore called a _______ microscope. -Answer-compound An image is produced when light is absorbed by a dark opaque object. The background has a bright white background. Your microscope is therefore also called a ________ microscope. -Answer-bright field The lenses nearest your eyes are the eyepieces or ______ lenses. -Answer-ocular What is the magnifying power of there lenses? -Answer-10x What is the collective name for the four lenses located nearest to the object being viewed? -Answer-objective lenses What is the name and magnification of the red banded lens? -Answer-4x scanning lens What is the name and magnification of the yellow banded lens? -Answer-10x low power lens What is the name and magnification of the blue banded lens? -Answer-40x high power lens What is the name and magnification of the white banded lens? -Answer-100X oil immersion lens Beginning with the light source, what 3 lenses of the compound light microscope does light pass through before it reaches your eye? -Answer-1. condenser lens 2. objective lens 3. eyepiece/ocular lens What is the mathematical definition of resolution power? -Answer-the shortest distance at which two objects can be visually separated The shortest visible wavelength of light is _____ nanometers (nm) -Answer-420nm Convert 420nm into micrometers -Answer-.42micrometers Bacteria is measured in _______ -Answer-micrometers Viruses are measures in _______ -Answer-nanometers What type of microscope is used to see viruses? -Answer-electron microscope What is the name of the smallest bacteria infecting humans at 0.2 micrometers? - Answer-Microplasma pneumonia What do you think your microscope is equipped with blue-violet sub-stage filter on the inside of the condenser? -Answer-because it's the smallest wavelength of light and it sharpens the image Define refractive index? -Answer-the number of degrees light rays are bent when passing through an object Immersion oil must have the same refractive index as ______ to be if any value. - Answer-glass Why do you think immersion oil is used between the slide and the 100x objective lens? - Answer-to get more detail What might happen if the slide is not securely placed, or the slide clip is released too fast? -Answer-it could fall out and break The ________ mechanical stage knob moves the stage front to back. -Answer-large The ____ mechanical stage knob moves the slide holder left to right. -Answer-small Parfocal means the image remains in focus after you have rotated the _____________________ from 4x to 10x. -Answer-revolving nosepiece As you increase magnification from 4x to 10x to 40x, what happens to the intensity of light that reaches your eyes? -Answer-it gets darker, you