Histopathologic Techniques Fixation, Chapter 3 Part I (Gregorios) Question and answers correctly solved 2023/2024

Histopathologic Techniques Fixation, Chapter 3 Part I (Gregorios) Question and answers correctly solved 2023/2024 Fixation - correct answers the first and most critical step in histotechnology involves fixing and preserving fresh tissue for examination first aim: preserve cell (life-like) second aim: harden and protect tissues most important: stabilization of proteins Additive fixation and Non-additive fixation - correct answers the two basic mechanisms involved in fixation Additive fixation - correct answers the chemical constituents of the fixative is taken in and becomes part of the tissue by forming cross-links or molecular complexes and giving stability to the protein e.g., formalin, mercury, and osmium tetroxide Non-additive fixation - correct answers fixing agent is not incorporated into the tissue, but alters the tissue composition and stabilizes the tissue by removing the bound water attached to H-bonds of certain groups within the protein molecule e.g., alcohol fixatives pH 6 and 8 - correct answe