b
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3
GCE
Wednesday 7 June 2023
Pape
9BN0/01
Afternoon (Time: 2
r
reference
hours)
Biology A (Salters Nuffield)
Advanced
PAPER 1: The Natural Environment and
Species Survival
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• appropriate. – there may
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Information
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•• The total mark for this paper is 100.
The marks for each question are shown in
– use this as a guide as to how much time to spend on each
• question. You may use a scientific calculator.
• brackets
In questions marked with an asterisk (*), marks will be awarded for your ability to
structure your answer logically showing how the points that you make are related or
follow on from each other where appropriate.
Advice
• Read each question carefully before you start to answer it.
•• Try to answer every
Check your answers if you have time at the end.
Turn over
question.
1
, Answer ALL questions.
Some questions must be answered with a cross in a box . If you
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change your mind about an answer, put a line through the box and
then mark your new answer with a cross .
1 Homo sapiens is the only living species of the genus Homo. Over 100 000
years ago, Homo neanderthalensis, a species closely related to modern
humans, lived with other Homo species in the same habitat.
(a) Which of the following is the correct definition of the term habitat?
(1)
A a group of organisms of different species living in the same area
B a group of organisms of the same species living in the same
area
C a place where an organism lives
D the role of an organism
AREA
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(b) A fossil bone discovered in China is thought to be from a new species,
Homo longi.
(i) Samples of DNA from this bone and bones from other Homo species
can be amplified using PCR.
Describe how these amplified samples of DNA can be prepared for (3)
analysis using gel electrophoresis.
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
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. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
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. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
AREA
DO NOT WRITE IN THIS
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2
, (ii) Explain how the results of this analysis would show whether Homo
longi is closely related to other Homo species.
(2)
DO NOT WRITE IN THIS
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. .. . .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
AREA
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(Total for Question 1 = 6 marks)
DO NOT WRITE IN THIS
AREA
DO NOT WRITE IN THIS
AREA
3
Turn
over
, 2 Human activities are affecting many habitats.
These activities can change the biodiversity of communities and populations
AREA
DO NOT WRITE IN THIS
within a habitat.
(a) Which of the following describes biodiversity within a habitat?
(1)
A the number of different alleles and the number of the same
species
B the number of different species and the number of different
alleles
C the number of different species and the number of the same
alleles
D the number of the same alleles and the number of the same
species
(b) Brown Moss is a site of special scientific interest. It is an area containing
shallow ponds, inhabited by birds and rare plants.
The biodiversity of plants growing in two ponds, A and B, at Brown Moss
was studied. The table includes data collected from the two ponds.
AREA
DO NOT WRITE IN THIS
Number (n) n(n–1) for Number (n) n(n–1) for
Species in pond A pond A in pond B pond B
Azolla filiculoides 5 20 0 0
Lemna minuta 29 812 0 0
Lemna trisulca 50 2 450 0 0
Alisma plantago-aquatica 2 2 0 0
Alopecurus aequalis 11 110 0 0
Carex pseudocyperus 2 2 0 0
Equisetum fluviatile 0 0 71 4 970
Juncus effusus 0 0 60 3 540
Menyanthes trifoliata 16 240 0 0
Polygonum amphibium 0 0 25 600
Ranunculus circinatus 13 0 0
Sparganium erectum 3 6 0 0
Typha latifolia 5 20 0 0
Total 156 9 110
4
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3
GCE
Wednesday 7 June 2023
Pape
9BN0/01
Afternoon (Time: 2
r
reference
hours)
Biology A (Salters Nuffield)
Advanced
PAPER 1: The Natural Environment and
Species Survival
You must have: Total Marks
Scientific calculator, HB pencil,
ruler
Instructions
•• Use black ink or ball-point pen.
Fill in the boxes at the top of this page with your
centre number and candidate number.
•• Answer
name,
all
Show all your working out in calculations and include units where
• appropriate. – there may
Answer the questions
questions.
be spaces
in the more space than you need.
Information
provided
•• The total mark for this paper is 100.
The marks for each question are shown in
– use this as a guide as to how much time to spend on each
• question. You may use a scientific calculator.
• brackets
In questions marked with an asterisk (*), marks will be awarded for your ability to
structure your answer logically showing how the points that you make are related or
follow on from each other where appropriate.
Advice
• Read each question carefully before you start to answer it.
•• Try to answer every
Check your answers if you have time at the end.
Turn over
question.
1
, Answer ALL questions.
Some questions must be answered with a cross in a box . If you
AREA
DO NOT WRITE IN THIS
change your mind about an answer, put a line through the box and
then mark your new answer with a cross .
1 Homo sapiens is the only living species of the genus Homo. Over 100 000
years ago, Homo neanderthalensis, a species closely related to modern
humans, lived with other Homo species in the same habitat.
(a) Which of the following is the correct definition of the term habitat?
(1)
A a group of organisms of different species living in the same area
B a group of organisms of the same species living in the same
area
C a place where an organism lives
D the role of an organism
AREA
DO NOT WRITE IN THIS
(b) A fossil bone discovered in China is thought to be from a new species,
Homo longi.
(i) Samples of DNA from this bone and bones from other Homo species
can be amplified using PCR.
Describe how these amplified samples of DNA can be prepared for (3)
analysis using gel electrophoresis.
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... . . .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. . . .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
AREA
DO NOT WRITE IN THIS
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... . . .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
2
, (ii) Explain how the results of this analysis would show whether Homo
longi is closely related to other Homo species.
(2)
DO NOT WRITE IN THIS
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. .. . .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
AREA
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. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. . .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. ..
. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. .. ... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. ... .. .. .. .. .. .. .. .. ......................
(Total for Question 1 = 6 marks)
DO NOT WRITE IN THIS
AREA
DO NOT WRITE IN THIS
AREA
3
Turn
over
, 2 Human activities are affecting many habitats.
These activities can change the biodiversity of communities and populations
AREA
DO NOT WRITE IN THIS
within a habitat.
(a) Which of the following describes biodiversity within a habitat?
(1)
A the number of different alleles and the number of the same
species
B the number of different species and the number of different
alleles
C the number of different species and the number of the same
alleles
D the number of the same alleles and the number of the same
species
(b) Brown Moss is a site of special scientific interest. It is an area containing
shallow ponds, inhabited by birds and rare plants.
The biodiversity of plants growing in two ponds, A and B, at Brown Moss
was studied. The table includes data collected from the two ponds.
AREA
DO NOT WRITE IN THIS
Number (n) n(n–1) for Number (n) n(n–1) for
Species in pond A pond A in pond B pond B
Azolla filiculoides 5 20 0 0
Lemna minuta 29 812 0 0
Lemna trisulca 50 2 450 0 0
Alisma plantago-aquatica 2 2 0 0
Alopecurus aequalis 11 110 0 0
Carex pseudocyperus 2 2 0 0
Equisetum fluviatile 0 0 71 4 970
Juncus effusus 0 0 60 3 540
Menyanthes trifoliata 16 240 0 0
Polygonum amphibium 0 0 25 600
Ranunculus circinatus 13 0 0
Sparganium erectum 3 6 0 0
Typha latifolia 5 20 0 0
Total 156 9 110
4
