MMSC407 Final Exam Questions with Correct Answers

MMSC407 Final Exam Questions with Correct Answers Describe the characteristics of light as an energy source. - Answer-1. Wavelength - the distance between the crests of two successive waves 2. Amplitude - the height of each wave 3. Frequency - the number of waves which pass an observation point in one second In terms of energy: -High energy = high frequency, short wavelength -Low energy = low frequency, long wavelength Describe the relationship between percent transmittance and absorbance. - Answer-A = 2 - log %T Beer's Law - how to calculate with it and how to interpret what it means in terms of the patient's results. - Answer-Beer's Law (1852) - absorbance is directly proportional to the concentration of the absorbing substance in the solution ∙Atotal = A1 + A2 + A3 ... ∙Cu = (Au/Astd) x Cstd where: →C = Cu = Cunknown = unknown concentration of absorbing substance (analyte) in solution →Au = Aunknown = absorbance of absorbing substance (analyte) in solution →Astd = Astandard = absorbance of the standard solution →Cstd = Cstandard = concentration of the standard solution Describe the parts of the spectrophotometer. - Answer-Basic components: 1. Source of radiant energy (light source) 2. Monochromator 3. Sample container (cuvette) 4. Detector system 5. Readout device Contrast the operational properties of the various types of monochromators reviewed. - Answer-1. Glass filter: colored glass, bandpass ~50 nm (wide) of radiant energy, inexpensive 2. Interference filters: monochromatic light produced by constructive interference of radiant energy, bandpass 5-10 nm (narrow) 3. Glass prism: non-linear, curved spectrum produced, rotated to allow desired wavelength through entrance slit