MIP 302, Final Lab Exam fully solved graded A+ 2023/2024

MIP 302, Final Lab ExamWhat is the appropriate attire for lab? - correct answer lab coats, closed toe shoes, goggles What are the five kingdom classification scheme? - correct answer 1. Animalia- animals 2. Plantae- plants 3. Fungi- molds & yeast (eukaryote) 4. Protista- protozoa & algae (eukaryote) 5. Monera- bacteria (prokaryote) What does the protist, Algae look like? - correct answer bc its photosynthetic and appear green or yellow and have rigid cell walls and symmetrical What does the protist, Protozoa look like? - correct answer motile, and photosynthetic produce enzymes that degrade cellulose What does the fungi, Yeast look like and act? - correct answer oval shaped, composed of chitin, reproduce asexually by budding and sexually by spore formation What happens with yeast under anaerobic conditions? - correct answer yeast will produce alcohol and CO2 by fermentation What does mold (fungi) look like? - correct answer long thread like filaments (hyphae) and join together to form myecelium free spores can be green or black What are the types of ocular magnifications? - correct answer 10x, 40x, 100x (w/oil immersion) How do you calculate the TOTAL magnification? - correct answer 100 X the magnification ex. 100 X 40= 400 total magnification What are the parts of the compound microscope? - correct answer How do you calculate resolving power? - correct answer RP= (0.5) wavelength/ NA NA- numerical aperture ex.) average wavelength of 550 nm and the low powered (10x), which has a NA of 0.25, the smallest object that can be clearly seen is 1.1 m RP= (0.5)550/ 0.25= 1100 nm What type of tryptic soy medium is it?q - correct answer all- purpose medium What is all purpose medium? - correct answer supports the growth of bacteria that don't have special nutritional needs What does tryptic soy broth look like? - correct answer turbid/cloudy--> shows if growth has occured What is tryptic soy agar used for? - correct answer isolation What are the four types of colony shapes? - correct answer circular, irregular, rhizoid (looks like lines all over), punctiform (small dots) What are the four types of colony textures? - correct answer rough, shiny, wrinkled, dry, dull What are the four colony margins? - correct answer entire (smooth edges) erose (sharp edges) filamentous (flagella) lobate (looks like cloud) What are the steps to */Gram Staining/*?? - correct answer 1. primary stain: crystal violet (1 min)