Histotechnology- Decalcification fully solved graded A+ 2023/2024

Histotechnology- Decalcification2. Acid solutions act as decalcifies by removing which of the following salts? a. calcium b. lithium c. potassium d. sodium - correct answers calcium 1. The effect of acid decalcification is most noticeable in the staining of: - correct answers Nuclei 3. After completion of decalcification, the specimen should be rinsed in: - correct answers Water 4. The amount of time a bone needs to remain in the decalcifying solution does not depend on: a. density of bone b. solution strength c. processing schedule d. temperature - correct answers processing schedule 5. Which of the following is a chelating agent? a. ethylene diamine tetracetic acid b. phenol c. hydrochloric acid d. nitric acid - correct answers ethylene diamine tetracetic acid 6. To offset the hydrolysis of nucleic acids caused by decalcifying agents, fix bone marrow in: - correct answers Zenker 7. Which of the following methods is not used to test for completion of decalcification? a. chemical b. electrolysis c. physical d. radiographic - correct answers electrolysis 8. In which of the following methods does decalcifying solution not have to be changed? a. acid b. calcium oxide c. chelation d. ion-exchange - correct answers ion-exchange 9. During decalcification, what gas is released? a. ammonia b. carbon dioxide c. nitrous oxide d. oxygen - correct answers carbon dioxide 10. Which decalcification method may cause burning of the specimen? - correct answers Electrolysis 11. Four factors that influence the rate of decalcification are: - correct answers Size of specimen, amount of solution, bone morphology, concentration of solution 12. For proper decalcification, bone should be cut at __________________ thickness. - correct answers 4-5mm 13. For proper cellular preservation, bone should be ___________________ before decalcification. - correct answers Fixed 14. The three methods to determine the end-point of decalcification are: - correct answers Physical, Chemical, Radiographic 15. Acid decalcifiers act by forming soluble calcium salts; chelating agents act by - correct answers absorbing calcium ions 16. Four methods of decalcification are: - correct answers Acid used alone, ion-exhcnage, electrolytic, ad chelation spongy (cancellous) bone - correct answers Inside the marrow, quicker decalcification dense/compact bone - correct answers Cement-like density outside of the bone, longer to decalcify decalcification - correct answers Removal of calcium from bone or other tissue