Histotechnology: H&E Staining questions & answers graded A+ 2023/2024

Histotechnology: H&E Staining the study of the microscopic structure of tissues what is histology? 1. to determine the cause of disease 2. determine the mechanism by which disease develops 3. to see the structural alterations of cells 4. to determine the consequences of the cell's alteration what are 4 ways a pathologist can use histology? so we can see all the components of the cell and the relation of cells to one another why do we stain tissues on slides? to differentiate between nucleus and cytoplasm what are routine stains used for? to demonstrate specific elements within tissue (such as connective tissue, muscle, lipids, nerves) what are special stains used for? to show nerves or some microorganisms what are heavy metal stains used for? deposition of metallic substance into the target cells how do heavy metal stains works? H&E (hematoxylin & eosin) what is the most common routine stain? to distinguish nucleus from cytoplasm and as a triage stain to distinguish normal from pathologic what is H&E stain used for? purple (hematoxylin) what color will the nucleus stain? pink (eosin) what color will the cytoplasm stain? purple what color will the ribosomes stain? pink what color will the cilia stain?