Histotechnology Mid Term questions with correct answers 2023/2024

Histotechnology Mid TermWhen is H&E QC done? - correct answers -Daily -Before any patient slides are stained What is H&E QC? - correct answers -Slides are checked manually for approval -Documented and logged for future inspections What is the meniscus? - correct answers -Bottom line of the curved surface of the liquid How many grams are needed to make 500mL of the 10% solution? - correct answers % desired:100 = g needed:volume desired -50g What causes tissue to shrink, dry, brittle - correct answers Too long in any steps: 1. fixation 2. dehydration 3. clearing 4. infiltration How do you correct air bubbles trapped during embedding? - correct answers -Tap cassette/block prior to placing it on the cold plate What is unique about clearing agents? - correct answers -They are miscible with both dehydrants and infiltrating agent -Xylene is able to interact with alcohol and paraffin What are the things that impact tissue processing? - correct answers -Length of time in reagent -Tissue type and density -Tissue thickness Decalcification methods - correct answers -Acid method (simple acid, ion exchange, electrolytic method) -Chelating method (EDTA) What are the acid methods of decalcification? - correct answers -Simple acid -Ion exchange -Electrolytic method (best) What is the chelating method of decalcificaiton? - correct answers -EDTA What is FormiCAL? - correct answers -Decalcification method -Uses formic acid (simple acid decal) and EDTA (chelatign decal) How should bone/hard specimens be embedded? - correct answers -On a diagonal -Smallest portion of the specimen should hit the blade first Three types of dehydrating agents - correct answers -Alcohol -Acetone -Universal solvent What is a universal solvent? - correct answers -Acts as both a dehydrating agent and a clearing agent -Ex: dioxane Best way to dispose of sharps? - correct answers -Biohazard sharps container What is the average temp for a cryostat? - correct answers -negative 20'C