Histotechnology Practicum: Test 1 questions & answers graded A+ 2023/2024

Histotechnology Practicum: Test 1Biopsy or Gross Specimen: small piece of tissue and derived from simple-natured procedure - correct answers biopsy biopsy or Gross specimen: whole organ and typically derived from surgeries - correct answers gross specimen What are the 10 steps in the pathology lab workflow? - correct answers 1. fixation 2. accession 3. grossing 4. continue fixation 5. dehydration 6. clearing 7. infiltration 8. blocking 9. section 10. staining what is the most important step in pathology procedures? - correct answers fixation what is the fixative to tissue ratio? - correct answers 20:1 when does fixation begin? - correct answers as soon as specimen leaves body what step is described as: matching specimen and requisition and assigning lab number - correct answers accession True or False: if a specimen doesn't have enough fixation, you are able to add more to the container - correct answers true what 3 identifiers must match on a requisition and label of specimen in order to be accepted? - correct answers 1. first name 2. last name 3. medicare number where is the lab number for specimens generated? - correct answers daybook what are the 3 duties of a fixative - correct answers 1. stop enzyme activity 2. kill microorganisms 3. firm specimen what is the most popular fixative - correct answers formalin routine processing takes how many hours? - correct answers 8-12 hours are alcohols considered a dehydrant, clearant or infiltrant? - correct answers dehydrant is xylol considered a dehydrat, clearant or infiltrant? - correct answers clearant what is the section thickness in microns for most specimens cut on a microtome? - correct answers 4 microns what is the difference between the workflow for a biopsy and the workflow for a gross specimen? - correct answers gross specimens requires 24 extra hours of fixation what is the difference between the workflow for fresh specimens and biopsies? - correct answers fresh specimens are STAT and are frozen and not processed. what are the turn around times for the following: biopsy, gross specimen, fresh tissue - correct answers -biopsy: 24 hours -gross specimen: 48 hours -fresh tissue: 10 minutes (then 24 hours because it is treated as a biopsy after) how long are requisitions kept for - correct answers indefinitely how long are wet tissue specimens kept for - correct answers 6 weeks after diagnosis how long are wax blocks and glass slides kept for? - correct answers indefinitely is freezing a form of preservation or fixation? - correct answers preservation what causes autolysis (self death) in tissue - correct answers lysosomal enzymes what causes putrefaction in tissue? - correct answers microorganisms is a preserved tissue able to be processed and sectionned? - correct answers no !! only fixed tissue true or false: fixatives kill the tissue - correct answers true excessive fixation may cause what? (2) - correct answers hardening and loss of compounds does increased temperature speed up or slow down fixation - correct answers speed up Above what temperature can injure tissue? - correct answers 45 degrees celcius