Histotechnology test 2 correctly answered rated A+ 2023/2024

Histotechnology test 2What is the consequence to improper clearing? - correct answers "Moth eaten" due to water still being in the tissues- improper dehydration Sections will crumble and fall out of block Shrink the centre of the tissue Why would a tissue shrink? - correct answers Wax is too hot What causes microscopic chatter? - correct answers Over dehydration seen most prominately at the ends of tissues What is the melting point do most paraffins? - correct answers 55-58 degrees celcius How should you dehydrate to minimize defects? - correct answers Graded alcohols increasing in concentration What is the most common disadvantage of xylene as a clearing agent? - correct answers Hardens tissues What causes cracks in a block? - correct answers Imbedding paraffin is too hot When is it possible to omit dehydration and clearing? - correct answers When the embedding media used is water soluable What is the purpose of fixing tissues? - correct answers To inhibit microbial growth, fix proteins and inhibit autolysis To preserve tissues in a life-like state. Hardens and fixes them for later steps What is infiltration? - correct answers Replacing water in tissues with wax What is embedding - correct answers Placing the tissue in a block of wax What must be done before staining? - correct answers Wax must be removed What reagents cause tissues to shrink? - correct answers Alcohol and xylene What could cause osmotic shock? - correct answers Rehydration What is eosin and what is it attracted to? - correct answers Acidic dye that's attracted to basic tissues What is the reasoning for the final dehydration step? - correct answers To prolong the tissue integrity What is grossing? - correct answers Tissues being examined by the pathologist or PA Examination includes specimen description then placing them into labelled cassettes What are the four steps of tissue processing. What is the purpose? - correct answers 1. Dehydration 2. Clearing (dehydration) 3. Infiltration 4. Embedding Purpose is the prepare the tissues for wax