CBE 205 Final fully solved graded A+ 2023/2024

CBE 205 Final Central dogma - correct answer -DNA to RNA to Protein -Replication-Transcription-Translation Watson and crick base pairing - correct answer -DNA: Adenine (A) to Thymine (T), Guanine(g) with cytosine (C) -RNA only change is A to U Replication - correct answer -Copying DNA -DNA polymerase catalyse the addition of DNA (deoxynucleotides) DNA -Primer is need to start new strand Direction of DNA synthesis - correct answer - 5' to 3' Major enzymes in DNA replication - correct answer -DNA polymerase -Primase -Helicase -DNA ligase Primase - correct answer -Synthesize a short stretch of RNA that is complementary to the template DNA -Ends with 3'-OH group - Allowsd DNA polymerase to work Replication Fork - correct answer -Where helicase separates dbl stranded DNA in to seprate strands for synthesis -End of the strand is okazaki fragments Helicase - correct answer -Unwinds double stranded DNA so it can be synthesized Okazaki fragments - correct answer -Lagging strands -DNA fragments that have been newly synthesized DNA DNA polymerase I - correct answer -exonuclease activity -Can degrade RNA primer (primase) DNA ligase - correct answer -Seal nicks in DNA that have adj 5'-PO4^2- and 3'-OH groups Origin of replication in bacteria - correct answer -Only place where the replication of DNA can start Transcription - correct answer -RNA synthesis -No need for a primer DNA - correct answer -Contains deoxyribose sugar -Contains base thymine (T) -Double stranded -One type RNA - correct answer -Contains ribose sugar -Contains the base Ucracil (U) -NOT double stranded -Multiple types Types of RNA - correct answer - Messenger RNA (mRNA) -Transfer RNA (tRNA) -Ribosomal RNA (rRNA) -small interfering RNA