WGU C785 Biochemistry Final Exam 2022/2023 Graded A+

3 functions of insulin: - 1. stimulates gluclose uptake 2. inhibits glycogen breakdown 3. stimulates fatty acid production and storage 3 Steps of PCR - 1. Denaturation: DNA is heated to 95C to separate it. 2. Annealing: reaction is cooled to 50C; primers stick to the DNA you want to copy and add DNA polymerase. 3. Elongation: reaction heated to 70C and DNA polymerase, adding nucleotides building a new DNA strand. 4 steps of Beta Oxidation: - 1. FAD -> FADH2 2. H2O 3. NAD+ -> NADH 4. CoA 5' AAG CGG TAC GTA 3' What would be complimentary strand? - 5' TAC GTA CCG CTT 3' 5' ATG AGT CTC TCT 3' What would be the mRNA sequence? - 5' AUG AGU CUC UCU 3' 5' ATG AGT CTC TCT 3' What would be the template strand? - 3' TAC TCA GAG AGA 5' 5'ATG AGT CTC TCT 3' What is the corresonding mRNA sequence? - 5'AUG AGU CUC UCU 3' This sequence is the same as the coding strand except T changes to U because it is RNA. RNA doesn't have T. 5'ATG AGT CTC TCT 3' Find the DNA template strand. - 3'TAC TCA GAG AGA 5' The DNA template strand is complimentary. So start with the opposite number, then go L-R with the complimentary letter. A dehydration reaction _______ a polypeptide, whereas a hydrolysis reaction ______ a polypeptide. - A dehydration reaction builds a polypeptide, whereas a hydrolysis reaction breaks a polypeptide.