CBSPD Sterilization - Certification Exam
Review – 91 Q’s and A’s
Steam Using Gravity Displacement: Method of Killing - -Denatures microorganism proteins -Steam Using Gravity Displacement: Sterilant Used - -Combination of steam and ambient air -Steam Using Gravity Displacement: How do steam and ambient are sterilize? - -Steam displaces the air, and forces it out of the drain vent. Steam then comes in direct contact with items -Steam Using Gravity Displacement: Parameters (3) - -1. Time
2. Temperature
3. Pressure -Steam Using Gravity Displacement: Common cycle times (3) - -1. 30 min, 250°F, 15 psi
2. 15-25 min, 275°F, 27 psi
3. 10 min, 275°F, 30 psi -Steam Using Gravity Displacement: Items that CAN be sterilized - -1. Critical items that are heat and moisture resistant
2. Semi-critical items that are heat and moisture resistant
3. Sharps Containers -Steam Using Gravity Displacement: Items that CANNOT be sterilized - -
Heat and moisture sensitive materials -Steam Using Gravity Displacement: Biological Indicator - -Geobacillus Stearothermophilus -Steam Using Dynamic Air Removal: Method of Killing - -Denatures microorganism proteins -Steam Using Dynamic Air Removal: Sterilants Used (3) - -1. Combination of
steam and ambient air
2. Prevacuum Sterilizers
3. (SFPP) Steam-flush pressure-pulse sterilizers -Steam Using Dynamic Air Removal: Parameters (3) - -1. Time
2. Temperature
3. Pressure -Steam Using Dynamic Air Removal: Common Cycle Times (2) - -1. 4 min, 270°F, 28-30 psi
2. 3 min, 275°F, 28-30 psi -Steam Using Dynamic Air Removal: Items that CAN be sterilized - -1. Critical items that are heat and moisture resistant
2. Semi-critical items that are heat and moisture resistant
3. Sharps Containers -Steam Using Dynamic Air Removal: Items that CANNOT be sterilized - -
Heat and moisture sensitive items -Steam Using Dynamic Air Removal: Biological Indicator - -Geobacillus Stearothermophilus -EO: Method of Killing - -Alkylation: interferes with microorganism metabolism -EO: Sterilants Used (2) - -1. 100% EO (undiluted) - common
2. EO Mixtures (contains HCFC and CO2) - uncommon -EO: Parameters (5) - -1. Time
2. Temperature
3. Pressure
4. Humidity
5. Concentration -EO: Common cycle time - -2.5 hours (not including aeration time) -EO: Items that CAN be sterilized (3) - -1. Critical items that are heat, pressure, and moisture sensitive
2. Semi-critical items that are heat, pressure, and moisture sensitive
3. Devices with long lumens -EO: Items that CANNOT be sterilized - -Trays with silicone mats (must follow manufacturer's guidelines) -EO: Biological Indicator - -Bacillus Atrophaeus -Gas Plasma: Method of Killing - -Interferes with microorganism metabolism by compromising essential components (enzymes, nucleic acid, etc.) -Gas Plasma: Sterilant Used - -Combination of hydrogen peroxide vapor and
gas plasma -Gas Plasma: Parameters (3) - -1. Time
Review – 91 Q’s and A’s
Steam Using Gravity Displacement: Method of Killing - -Denatures microorganism proteins -Steam Using Gravity Displacement: Sterilant Used - -Combination of steam and ambient air -Steam Using Gravity Displacement: How do steam and ambient are sterilize? - -Steam displaces the air, and forces it out of the drain vent. Steam then comes in direct contact with items -Steam Using Gravity Displacement: Parameters (3) - -1. Time
2. Temperature
3. Pressure -Steam Using Gravity Displacement: Common cycle times (3) - -1. 30 min, 250°F, 15 psi
2. 15-25 min, 275°F, 27 psi
3. 10 min, 275°F, 30 psi -Steam Using Gravity Displacement: Items that CAN be sterilized - -1. Critical items that are heat and moisture resistant
2. Semi-critical items that are heat and moisture resistant
3. Sharps Containers -Steam Using Gravity Displacement: Items that CANNOT be sterilized - -
Heat and moisture sensitive materials -Steam Using Gravity Displacement: Biological Indicator - -Geobacillus Stearothermophilus -Steam Using Dynamic Air Removal: Method of Killing - -Denatures microorganism proteins -Steam Using Dynamic Air Removal: Sterilants Used (3) - -1. Combination of
steam and ambient air
2. Prevacuum Sterilizers
3. (SFPP) Steam-flush pressure-pulse sterilizers -Steam Using Dynamic Air Removal: Parameters (3) - -1. Time
2. Temperature
3. Pressure -Steam Using Dynamic Air Removal: Common Cycle Times (2) - -1. 4 min, 270°F, 28-30 psi
2. 3 min, 275°F, 28-30 psi -Steam Using Dynamic Air Removal: Items that CAN be sterilized - -1. Critical items that are heat and moisture resistant
2. Semi-critical items that are heat and moisture resistant
3. Sharps Containers -Steam Using Dynamic Air Removal: Items that CANNOT be sterilized - -
Heat and moisture sensitive items -Steam Using Dynamic Air Removal: Biological Indicator - -Geobacillus Stearothermophilus -EO: Method of Killing - -Alkylation: interferes with microorganism metabolism -EO: Sterilants Used (2) - -1. 100% EO (undiluted) - common
2. EO Mixtures (contains HCFC and CO2) - uncommon -EO: Parameters (5) - -1. Time
2. Temperature
3. Pressure
4. Humidity
5. Concentration -EO: Common cycle time - -2.5 hours (not including aeration time) -EO: Items that CAN be sterilized (3) - -1. Critical items that are heat, pressure, and moisture sensitive
2. Semi-critical items that are heat, pressure, and moisture sensitive
3. Devices with long lumens -EO: Items that CANNOT be sterilized - -Trays with silicone mats (must follow manufacturer's guidelines) -EO: Biological Indicator - -Bacillus Atrophaeus -Gas Plasma: Method of Killing - -Interferes with microorganism metabolism by compromising essential components (enzymes, nucleic acid, etc.) -Gas Plasma: Sterilant Used - -Combination of hydrogen peroxide vapor and
gas plasma -Gas Plasma: Parameters (3) - -1. Time
