SBB Exam Practice Questions and Answers Latest Updated 2023/2024 | 100% Verified

SBB Exam Practice Questions and Answers Latest Updated 2023/2024 | 100% Verified. Plasma manufacturers can use donors positive for what? - Anti-HBc Anti-HTLV-I/II Cytapheresis and Auto donors must be tested for ID how often - Every 30 days reactive ID results must be... - tested in duplicate for confirmation Neutralization: HBsAg and HIV-1 Ag Western Blot - Anti-HIV RIBA - Anti-HCV Anti-HTLV confirmed by repeating the test using another manufacturers EIA test HTLV testing and deferral - Two+ positives: 2 donations by same manufacturer = indefinite deferral 1+ by one manuf. and 1+ by another manuf = . 1+ by one manuf. and negative by another manuf. = 8 week deferral What does Dextrose in additive do for cells? - supports ATP generation What does Adenine in additive do for cells? - provides substrate for ATP synthesis What does sodium biphosphate in additive do for cells? - controls pH What does citrate in additive do for cells? - prevents coagulation What is the storage time if additive used is CPD, CP2D? - 21 days What is the storage time if additive used is CPDA-1? - 35 days What is the storage time if additive used is AS-1 (Adsol)? - 42 days What is the storage time if additive used is AS-3 (nutricel)? - 42 days What is the storage time if additive used is AS-5 (Optisol)? - 42 days Whole Blood storage temp and description - 1-6C contains all blood elements plus the anticoagulant-preservative commonly used as a source for component production RBC storage and description - 1-6C Whole blood with plasma removed RBCs without additives must have final hct <80% Frozen RBC storage and description - -65C or colder frozen within 6 days if no additive solution RBC w/additive must be frozen before expiration must be put in freezer within 4 hours of removed from 4C Rejuvenated RBC description - rejuvenation should restore 2,3-DPG to 75% of normal level and ATP above normal levels. If done before 14 days may cause high 2,3-DPG which impairs O2 up-take. AS-3 and AS-5 cannot be rejuvenated Deglyced RBCs storage and description - 37C warmer or waterbath (20-25 minutes) washed with solutions of decreasing osmolarity to avoid hemolysis. must remove cryoprotectant agents, minimial free hgb, and result in recovery of at least 80% RBC volume. open system:1-6C with 24 hr expiration. closed system: 14 days or as FDA approved What should the osmolality of a deglyced unit be? - <500. Osmolality is a fxn of free glycerol therefore an increase is a result of improper deglycing What should free hgb in last wash be from decglyed unit? - <200 Washed RBCs storage - 1-6C washed with sterile normal saline to remove most of plasma 24 hour expiration FFP storage and description - prepared from WB or collected using apheresis. frozen at -18C or below contains max levels of labile or nonlabile coag factors Thaw between 30-37C Thawed plasma - FFP thawed but not transfused within 24 hrs can be stored 1-6C for 5 days reduction in Factors V and VII Liquid plasma - plasma separated from WB at any time during storage, up to 5 days after the expiration date of WB. supernatant levels of potassium and ammonia are increased Cryo - prepared from WB collected into CPDA-1 or CPD precipitates when FFP thawed between 1-6C stored at -18C or colder suspended in less than 15mL plasma contains factor VII and fibrinogen and most of XIII single unit store at 20-24 for 6 hrs pooled store at 20-24 for 4 hours platelets - prepared from WB that hasn't cooled below 20C separated within 4 hours soft spin (plt rich plasma) followed by a hard spin (platelet concentrate) resuspended in about 45-65mL plasma 20-24C with gentle agitation Granulocytes - usually harvested in buffy coats collection must yield 1.0x10 to the 10th granulocytes Transfuse ASAP Irradiation - prevents proliferation of transfused T-Lymphs, the primary cause of graft vs host (GVHD) effects of storage on blood components - decreases 2,3-DPG levels: this controls release of O2 from RBCs, levels recover after 24 hrs post txn decreases ATP increases potassium Leukocyte reduction must reduce WBC count to what? - <5x10 to the sixth in at least 95% of units samples and yield >85% RBC recovery What must the minimum mean volume of Apheresis RBCs be? - 180mL packed RBC volume per unit (>60g hgb) 95% of samples units must have at least 150mL (>50g hgb) What must the minimum mean volume of Apheresis RBCs leukoreduced be? - <5x10 to the sixth WBC and mean volume of 153mL packed RBC volume per unit (>51g Hgb) 95% of sample units have at least 128mL (>42.5g hgb) How much coag factor VIII and fibrinogen must cryo have? - 80IU Factor VIII and 150mg fibrinogen pooled units must have same amt multipled by number of units in pool What are Mendel's principles? - random segregation: distinct genes are randomly inherited one from each parent what is independent assortmant (Mendel's principle)? - genes inherited independently if carried on different chromosomes. combos of genes are not dependent on other genes (exception: linkage) what is an autosome? - any chromosome other than the sex chromosomes What is meiosis? - process by which sex cells are produced which have only one copy of each chromosome (haploid). What is mitosis? - process where sex cells are duplicated and equally divided between two daughter cells zygote - fertilized egg gamete - sex cells....egg and sperm diploid - normal number of chromosomes in zygote and all body cells (46) haploid - normal number of chromosomes in all gametes (23) structural gene - gene that controls the protein production of another gene or genes regulator gene - gene that controls the rated of production of another gene or genes allele - alternate forms of a gene at a given locus locus - location of a gene on a chromosome linkage - two loci carried on the same chromosome and usually inheritied together. The closer the loci, the closer the linkage. Seen in Rh, MNS and HLA codominant - both expressed in heterozygous state. Ex: AB, Fy(a+b+) dominant - trait expressed in homo or het state recessive - trait only expressed in homo state propositus - member of a family from whom the whole family came to be investigated Chromosome 1 contains which blood groups - RH, Scianna, Duffy, Cromer, Knops Chromosome 4 contains which blood groups - MNS Chromosome 7 contains which blood groups - kell, Colton, YT Chromosome 9 contains which blood groups - ABO Chromosome 18 contains which blood groups - Kidd Y linked - all sons will be affected. passed on Y from fathers to sons X linked recessive - affects mostly males, females usually carriers, passed from mother to son, never passed directly from father to son, affected males may be seen to skip generations autosomal recessive - trait usually only appears in sibs, on average 1/4 of the sibs of the propositus are affected, parents may be related autosomal dominant - trait appears in every generation transmitted by an affected person to half his children unaffected persons do not transmit the trait to their children X-linked dominant - affected males transmit trait to all daughters and to none of sons. affected females pass to half their kids Hardy and Weinberg formula - (p+q)2 I DON"T UNDERSTAND THIS...ASK PAM To determine allele frequency of two or more unrelated antigens, multiply frequencies of each trait - Ex: 70% E neg, 90% K0.7x0.9=0.63. So 63% E-KWhat enzymes do the ABO genes produce? - Glycosyltranferases. Precursor of the A and B antigens is the H antigen. ABO alleles are autosomal co-dominant